There is much confusion as to how stable Long R3IGF-1 is once reconstituted, however unlike wild-type IGF-1, Long R3IGF-1 should be stable for several months once reconstituted even at room temperature, but ideally kept at 4oC, if not -20oC (manufacturers of Long R3IGF-1 recommend reconstitution in 100mM acetic acid to 1mg/ml and stored at -20oC for up to 3 months). This stability is dependent upon correct reconstitution with 100mM acetic acid. The concentration of Long R3IGF-1 is usually at 1mg/ml, thus 0.04ml would be required for 40mcg (4IU on an insulin needle). I know people who have used IGF-1 after having kept it reconstituted in acetic acid in the freezer for 1 year and still got great results. If this is a concern though, one can purchase the lyophilised form of Long R3IGF-1, which can be stored at 2-8oC, and reconstitute themselves, however bear in mind this reconstitution must be done with a sterile solvent and using sterile techniques. Finding the actual studies for this has proven to be somewhat troublesome, as they don't exist publicly, but rather we have to rely on the information given by GroPep on their website. NB: GroPep are the company that produce LongR3 IGF-1, and also own the patent (see www.gropep.com.au for more information):
How long is the stock solution stable for under these storage conditions?
Liquid stability data shows that Long R3IGF-1 is stable for 3 years (-20°C to 37°C). Therefore, the stock solution should be stable at 4°C for 3 years.
Is Long R3IGF-1 stable?
Re-test date for freeze-dried peptide is 3 years. Liquid formulation stability studies have recently been completed. It is stable for 3 years (-20°C to +37°C). We have data indicating stability in media at 4°C for 1 year.
Please note, that when GroPep state 'liquid stability' they are referring to LongR3 IGF-1 that has been reconstituted by their recommended techniques, i.e. 100mM acetic acid to a stock concentration of 1mg/ml. Some websites sell media grade Long R3 IGF-1 in both lyophilised and reconstituted form, with the latter being reconstituted according to GroPep's recommendations (100mM acetic acid). If you state in your order from some reputable companies that you would like some extra acetic acid, they usually include an extra 5ml or so for free, so you can dilute your solution further if desired.