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12-09-2016, 04:40 AM #1
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How to choose right syringe filters and filter membranes
Hi guys,
The texture of syringe filters include Nylon, PVDF, PTFE, MCE, CA, Glass Fiber, PP, PES.
When choosing the texture of syringe filters, we need to consider pore diameter, chemical resistance( if can resist all carrier oils and solvents), oil-based or water-based solution, and etc.
Here are the pore diameter of common syringe filters.
Pore diameter:
Nylon, PVDF, PTFE, MCE, CA, PP, PES: 0.22um, 0.45um
Glass Fiber: 0.7um, 1.2um
Here are some common questions we enounter when choosing proper syringe filters?
1. Is it necessary to have a 0.22 um to have a sterile solution or the 0.45 um gives acceptable results concerning the dangers of bacteria ?
2. What is the average filtering time per 100ml oil-based injections and water-based injections? What carrier oil you use?
3. Warming your injection will make filtering much easier. What is the filtering time difference between warmed and unwarmed oil?
4. Which membrane are the best for a steroid based solution? Oil-based and water-based.
Here is the chemical resistance chart of Whatman syringe filters/filter membranes.
Nylon PVDF PTFE MCE CA Glass Fiber PP PES
Benzyl Alcohol LR R R R LR R R NR
R=Resistant
LR=Limited Resistance
NR=Not Resistant
I would like to answer the above questions with my shallow expertise.
1. 0.22 um is used for sterilizing-grade filtration. Literally speaking, getting rid of bacterials. 0.45um is used for clarification filtration and pre-filtration. Always stick to
0.22 um though more time-consuming. I want to correct my words in the past posts.
4. Just considering the pore diameter, Glass Fiber is out of the game. Then comes to chemical resistance, Nylone, CA, and PES are out of the game too. So the rest
options might be good for use: PVDF, PTFE, MCE, PP. But deeper consideration is: would these syringe filters not be eaten by other solvents(like BB, EO, guaiacol,
PS80), even carrier oils? As far as I know, sesame oil eats PES.
My provisional conclusion is: 0.22 um of PVDF, PTFE, MCE, PP are good for use. But it is a open proposition.
All MC bros are welcome to anwser these questions. Always glad to discuss this with all you guys.
PS:
Nylon: Nylon
PVDF: Polyvinylidene Fluoride
PTFE: Polyfluortetraethylene
MCE: Mixed Cellulose Ester
CA: Acetate Cellulose
Glass Fiber: Glass Fiber
PP: Polypropylene
PES: Polyether SulfoneBERSERKER973, pgb thanked for this postpgb liked this post
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12-09-2016, 05:00 AM #2
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Whoops. @presser. Please correct these mistakes for me. I can not reedit my post here.
1. How to chosee right syringe filters and filter membranes-- How to choose right syringe filters and filter membranes
2. Here are the pore diameter of common syringe filters.--Here is the pore diameter of common syringe filters.
3. 0.22 um of PVDF, PTFE, MCE, PP are good for use--0.22 um PVDF, PTFE, MCE, PP are good for use
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12-09-2016, 11:44 AM #3
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hmm, I literally just bought a few more nylon filters because I seemed to have read all over the web that was preferred.
I didn't quite understand the way you worded it. You said nylon and pes are out because of chemical resistance. What does that mean? That solvents will eat the filter?Chocolate Rain liked this post
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12-09-2016, 12:51 PM #4
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Thanks big Iron up for this thread and concerning my questions. So .22um PVDF and ptfe ate good, correct?
And solvents ba and bb with GSO what types of plastic or bottle tops that are resistant?
And if using glass media bottles can they be bought sterile that way I can transfer into sealed sterile vails with syringe.
I have brewed before but it been a while and used whatman .22 syringe filter into 50 ml veils. I used 60 ml syringe with calk gun and switch back and forth to Springs to hold pressure. Don't remember type of whatman I used I had followed a guide each time for everything.
I have vacuum electric and hand pump don't know if I should try bottle topm
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12-09-2016, 03:28 PM #5
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Correct I have read that PES definitely will melt and nylon from what I have read has to be only the ones that are chemical resistant they will bust easy and there is a plastic piece inside membrane that will melt. I read only certain types can handle heat and solvents like ba and bb. Like some of the color codes or for specific types of cultures and resistance. That is what I read.
Better to go with pdfv is what I have read.Chocolate Rain, PezBolic liked this post
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12-09-2016, 04:15 PM #6
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12-09-2016, 04:41 PM #7
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What kind is that? Relax let a mechanic teach u lol. Get some latex gloves pull them over from bottom and wrap with electric tape. That will give u a good seal.
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Also if u have a nebulizer it's easy to use it as a vacuum I can tell u how to do that too or make other electric vacs
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If u have any compressor u can create suction with intake with little modifications. U need a gauge and Valve to close once max vacuum pressure is created then u kick on when necessary or go check every once and while depending on leaks.
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The chemical resistance should be limited resistance as stated above (LR). Maybe go with less bb and ba. Go with minimal amount necessary. If u doing enathate there are different recipesbig_iron_up thanked for this postbig_iron_up liked this post
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12-09-2016, 05:39 PM #8
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12-09-2016, 06:45 PM #9
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U can get the sterile glove packs from pharmacy that way nothing can get into your mix. Sterile glove will cover surface area and where your top section is pull insert section of glove over that way tape touches nothing but glove. Remove with a razor knife.
For a vacuum pump nebulizer take intake plug out and remove filter. Find a hose to fit snug inside install a push on T. Then add section of hose to a vacuum gage. Next put a valve from T. Make sure gauge is between valve and bottle top.
To make a good seal u can find an o-ring to fit over hose and inside intake on nebulizer find o-ring thickness and diameter to give a snug fit on both hose and intake. When u ready open valve when u get to desired vac pressure shut off nebulizer and close valve. Watch gauge for next time to kick on. Or check now and then. If u make a good seal it will hold vacuum until no fluids are on top.
All modification parts can be purchased at a auto parts store. Gauge, valve, hose, o-rings, all of it.
Calk gun method with filter syringe is way I used to go. I have extra springs to put between for constant pressure. Make a stand to hold guns just above bottle 50 ml with filter and 60 ml syringe. I think it's 43 ml u can hold at time in syringe to fit gun. This way works and u don't have to but crank a little when tension runs out.
I am a mechanic and very mechanically minded and inclined. I hope this helps. Will do a thread on it all later when I have time.
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12-09-2016, 10:11 PM #10
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@trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...15100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um PVDF, PTFE, MCE, PP are good for use.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass bottle tops is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.
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@trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...1015100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um of PVDF, PTFE, MCE, PP are good for us.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass units is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.Muscle mechanic thanked for this postMuscle mechanic liked this post
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12-09-2016, 10:18 PM #11
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12-09-2016, 10:19 PM #12
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@trekrider215
Here is the link of Whatman chemical compatibility chart. Please check this out:
https://www.gelifesciences.com/gehcls...1015100012.pdf
LR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration.
NR means that PES is Not Resistant to BA. BA wil melt PES for sure.
@Muscle mechanic
Yes. 0.22 um PVDF, PTFE, MCE, PP are good for us.
For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass units is also good for use.
Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.Muscle mechanic thanked for this postMuscle mechanic liked this post
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12-09-2016, 10:22 PM #13
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Okay, thank you this was an excellent post. Cleared things up a lot for me.
Well, i'm running 100ml's through a nylon .22 Nalgene with glass media bottle because it was the only thing I bought because I was initially under the impression nylon was the way to go. So far it's going very well. just kind of slow. I'm sure your right that there is a cut off point in which it begins to melt the filter, this might explain my first batch.
@musclemechanic, I truly appreciate you guidance on how to rig my filter up. I tried to make a seal with a glove and duct tape but it still didn't seem to hold. I just don't understand how they can make a bottletop filter and say it's for a 45mm glass media bottle and not put fucking threads on the bottom of it. I'm calling medlabsupplys and making them send me a replacement. That's bullshit. I just don't understand how its supposed to hold a vacuum without a tight seal. I shouldn't have to rig it up. It's only a 12 dollar tool so no big deal, i'm sure they'll send a replacement. For the record this is the .22 Nylon Whataman Zapcap 500ml bottle top filter. If anyone reads this, don't buy one!
The Nalgene seems to be getting it done though. A few pumps of the hand vac and it's on its way. The glass medium bottle made a huge difference from the other night when I first tried because I can get much more suction with the glass media bottle and not worry about it cracking.
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My bad, I didn't mean to post the picture twice. It won't let me delete it. Anyways, you can see what a big difference it made. I have no idea why the gear up top looks like it came out of someones rotten kidneys lol.Muscle mechanic thanked for this postMuscle mechanic liked this post
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12-10-2016, 03:17 AM #14
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So after 6 hours lol, i was able to get about 90mls through the filter pictured up above. It got to a point where it literally just wouldnt drip at all and im pretty sure i may have even maxed the pressure out on the hand pump.
The filtered media came out literlly flawless, crystal clear. Theres still about 30-40 unfiltered i poured into a jar and will syringe filter tommorow.
Im a little suprised i couldnt get the full amount through it. I did notice obvious signs that the top cup was being eaten by the solvents, so i can only assume that somehow the internal working were damaged causing it to some how clog up. This may be the point big iron talked about.
I see no signs that any of it actually ended up in the geqr though. Oil is crystal clear.
Filter head pressure to the very end unlike the other filts i tried that i couldnt get it to hold for even a few minutes.
Had i got the bigger version of this filter i could have had twice the diameter of filter which i imagine woul have greatly increased the speed.
Pvdf will be my next order to compare. I think nylon and pes can be used you just have to pay attention. Its obvious this stuff chews up the plastic pretty well.
The first batch i made i did exactly as i did tonight with sane filter and everything and i now know the reason it cane out cloudy was because of the plastic media bottle i used then versus the glass media bottle I used tonight.
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You can actually see in the pic above. The top bottle cop, the hazy line above the oil. Thats not oil. That foggy area is the plastic being basically dissolved.
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12-10-2016, 09:12 AM #15
ok, I just went to the link you posted to see what the hell y'all were talking about, and who the fuck understands this shit lmao, Fuck man you need a Chemistry Degree to read that shit
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12-10-2016, 09:45 AM #16
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12-10-2016, 11:23 AM #17
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Ptfe. Seams best have looked more last night on Millipore says nylon and another site syringe filters say ptfe for bb.
They just say to use a prolypropylene housing and top going into glass. So brand may have alot to do with it. I will email a manufacturer and ask recommended type for bb and baPresser liked this post
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12-10-2016, 12:06 PM #18
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@trekrider, so I am gathering that the filter filters any plastic residue that melts from top section. So even prolypropylene will melt or be eaten up some?
Be specified of filter used and top with media bottle please. Brand, type, size, housing type(prolypropylene?), Pore size.
Your zapcap is supposed to create a seal on any media bottle standard size. That's odd that it wouldn't. It is compatible just as luer lock and luer slip. Talked to a buddy this morning who told me that's how zapcap seals and works. I never used just saying what he said.
I play dumb to anyone I know I just tell them someone asked on forum. I have never told a soul that I have brewed before or plan on doing it. People just talk too much shit! Lol
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12-14-2016, 02:15 AM #19
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Hey bro Maine Manufacturing makes the zapcaps now and I still love 'em. They are tougher than the Millipore steritops or cups [I had at least 5 leak on me and I've been doing this almost 15 yrs].
Nylon is your best all purpose. If you have 100% EO or want to filter someting like Guiacol so it's 'sterile' you'll want the 'PTFE' membrane (It's similar/partially teflon).
One gripe I get is that other than the cellulose acetate (just say no) zaps are NOT sterile. Easy fix. Have a bottle of 0.22 filtered BA and spray it on the filter surface. Attach vacuum source. Drip,drip,drip... NOW it's sterile ! Use a 0.45 micron first. NEVER, EVER be a tightass and start with a 0.22. You'll eat up filters and waste money. Ideally filtering thru a 0.8 micron and 0.45 micron nylon filter sheets (vacuum flask setup) is best but it takes looking.
Here's a trick. Have some textured gauze. When the flow slows down I suck the liquid out into glassware. I then clean the surface well and microwave the liquid to a reasonable (consult your reference temp for membrane). You will get about 750ml out of 500 -or should unless you are getting powders far downstream). Same goes for the 0.22 Nylon zapcap- I recommend a basic MCT 60/40 (c8/c10) mix. Nobody is getting 500 without pre-filtering. MAYBE with Digycol (840?) which has a viscosity of 10. Grapeseed oil is 54, CSO is 76.
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12-14-2016, 02:23 AM #20
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Hey bro Maine Manufacturing makes the zapcaps now and I still love 'em. They are tougher than the Millipore steritops or cups [I had at least 5 leak on me and I've been doing this almost 15 yrs].
Nylon is your best all purpose. If you have 100% EO or want to filter someting like Guiacol so it's 'sterile' you'll want the 'PTFE' membrane (It's similar/partially teflon).
One gripe I get is that other than the cellulose acetate (just say no) zaps are NOT sterile. Easy fix. Have a bottle of 0.22 filtered BA and spray it on the filter surface. Attach vacuum source. Drip,drip,drip... NOW it's sterile ! Use a 0.45 micron first. NEVER, EVER be a tightass and start with a 0.22. You'll eat up filters and waste money. Ideally filtering thru a 0.8 micron and 0.45 micron nylon filter sheets (vacuum flask setup) is best but it takes looking.
Here's a trick. Have some textured gauze. When the flow slows down I suck the liquid out into glassware. I then clean the surface well and microwave the liquid to a reasonable (consult your reference temp for membrane). You will get about 750ml out of 500 -or should unless you are getting powders far downstream). Same goes for the 0.22 Nylon zapcap- I recommend a basic MCT 60/40 (c8/c10) mix. Nobody is getting 500 without pre-filtering. MAYBE with Digycol (840?) which has a viscosity of 10. Grapeseed oil is 54, CSO is 76.
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12-14-2016, 02:55 AM #21
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Believe it or not the Foxx 0.45 PES can take 250mg (1% BA, 10% BB) enanthate in GSO or MCT oil like a it's a big gulp. I wouldn't rely on 0.22 but you'd probably be OK with it. I am not sure why nylon isn't rated higher. For chemikaze's using Guiacol or other potent solvents I used to say PVDF but Millipores bottle unit's IMO have been shitty whereas I have beaten the living dogshit out of Zapcaps and they overperform by 50%+ .
PTFE filters strong acids and & aldehydes.Muscle mechanic, big_iron_up liked this post
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03-23-2017, 11:21 PM #22
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03-28-2017, 11:30 PM #23
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03-29-2017, 07:48 AM #24
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05-12-2017, 11:45 PM #25
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.45 is considered sterile.
And I am pretty sure most labs use It!
Anyway they say certain virus's can get thru but u don't get I viral abscess only bacteria ones!
Ba will stop production of viruses and they will not be preserved in your vial to become active. They need a host or they die!
But still baking is done with. 45 and the time saved u can bake a cake and lots of stuff while oven us hot!
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05-16-2017, 12:16 AM #26
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.45 PVDF sterile Whatman.. Done. If you want to be there all day by all means use the .22. I have yet to come across anyone that has gotten an infection from using a .45. Maybe I'm just lucky when comes to filtering. Good luck
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08-15-2017, 10:18 AM #27
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These links are compatibilty charts. There are some things missing but you will get an idea. PES is only ok for a prefilter of a very low BA/BB mix. Nylon is the best overall but prefiltering ie a 0.8 or 1.0 micron nylon syringe filter or 47mm membrane (all found in nylon) are ideal if you want to save your filters. I am going to put a post dispel a lot of myths are things people are doing wrong.
https://sevierlab.vet.cornell.edu/res...art-Detail.pdf
https://www.treborintl.com/content/ch...sistance-chart
Pics (right) Millipore 0.22 micron PVDF STERIVEX. Prefiltered and using the veterinary syringe NOBODY SHOULD OR NEED TO USE A CAULK GUN. That syringe is $8 at any tractor supply or online. Guys, You've gotta prefilter. It is easier and saves you money. You can't be a cheapskate.
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08-15-2017, 01:45 PM #28
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One thing about this thread. I am fairly certain the ratings against Benzyl Alcohol,BB, Acetone,ect is if the ENTIRE sample is that chemical. I can see this from empirical experience. Nylon needs to be 'nylon 66' made material. It works. However, if one were filtering pure solvents PTFE is the only way to go. EO is falling way out of favor as far as I can tell and guiacol works but it stinks and it comes thru skin pores. Whomever is content with using 0.45 micron pore size remember staph aureus can routinely get thru this size. The link below even says 0.22 membranes can have this happen. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896367/
Oil and BA help a lot. Water is like a petri dish. Theoretically 0.1 micron filters would be as good as one could get but that is not practical. You have to be a search engine magician to find them in a syringe filter of a suitable membrane.
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08-30-2017, 07:28 AM #29
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Quick question
Can someone explain the differences between inlet outlet female male?
Getting some filters and they have female inlet and male outlet. Are these OK to use? I have PDVF 0.22um 33mm and PES 0.10um 33mm both sterile. The first used for oils and the second for amino acids in water.
Thanks
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09-16-2017, 01:18 PM #30
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Using a 90mm (or whatever diameter the membrane is) nylon or PVDF filter membrane ON top of say the 0.22 micron PES will do the dirty work and should fix this problem. Unless already pre-filtered a 0.65-0.45 micron size will do it. Just look at the drip. If it rolls through like the Wermacht thru Poland you are kind of screwed. PES ones that sell for $13 are not a good buy. Maybe if they are $5 each in a box of 12 (fire sale price) they can be useful. If someone is making a bunch of bacteriostatic water or filtering GSO, CSO, or MCT oil. It' s not a problem. Just my $0.02.
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09-16-2017, 01:22 PM #31
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09-16-2017, 10:29 PM #32
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Cellulose Acetate is not anywhere near ideal. If you had a 70mm membrane set on top of it that would help a lot. That's the size they come in. Hard to come across unless you look quite a bit. And never use Nalgene Nylon filters. They suck and Foxx bioscience is weak too (membrane is not very thick). At least with those they take 90mm membranes. Watch the flow rate. It should be a steady drip (quick) not like sucking Pepsi through a straw.
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09-16-2017, 10:45 PM #33
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The context needs to be made. Resistance is relative. Keep in mind this is referencing SRTAIGHT BA, BB, ect. so even the wimpy PES can handle some things in small amounts but I would avoid altogether. Nylon: It gets a bad rap. It's a good membrane as long as them membrane is adequately thick
.
PDFE - there are hydroPHILIC and hydroPHOBIC versions- that being said this is for filtering PURE SOLVENTS. You can use it but I can tell you this much, use it in syringe filters and get out a fucking sundial.
PVDF: All purpose, hydrophilic, and is good. Nylon is cheaper , and for the most part is perfectly ok. For a syringe use PVDF gets the nod.
MCE: I'm not sold on it. Have no feedback.
AS ALWAYS DO RESEARCH AND PREFILTER. DON'T SPEND $6.00 TO FILTER 30CC OR RUIN YOUR THUMBS. I have no idea why I see dudes wasting $$ using 0.22 only. YES-use them, but find a larger pore and you will have a much easier time. Spend a little to save a lot of money and effort. Hint -ebay.
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09-16-2017, 10:51 PM #34
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Sorry about the gigantic font. 47mm filters and the 300ml beaker top with metal clamps and receiver cost maybe $50. Find some and if you want sterile then use the syringe filters. For the cost of a 1.5 cfm 1/4 HP pump ($90 @ Harbor Freight) you are set. MCT oil is 1/2 the viscosity of GSO and by the gallon it's $40. I dont know who would need that much so start cooking with it and putting it in shakes.
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09-17-2017, 12:38 PM #35
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01-26-2019, 05:15 PM #36
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What is an inorganic filter membrane at .22 microns what type it's it?
nouler Juler 1000Ml Filter Unit Vacuum Unit + 50X 0.22Um Inorganic Filter Membrane for Filtration of Aqueous Solution Amazon.com: nouler Juler 1000Ml Filter Unit Vacuum Unit + 50X 0.22Um Inorganic Filter Membrane for Filtration of Aqueous Solution: Sports & Outdoors
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02-15-2019, 09:03 PM #37
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I am brand new to home brew. I have finally gotten all of the things I think I need. (I had to wait a good bit to get the glass rods for stirring)
I have syringe filters. They are supposed to be good up to 100mls, they are the 25mm size.
After continually reading I am concerned. Can I actually end up rupturing the membrane in the filter if I try to push it through with to much force? They are PVDF filters. The "large" volume syringes I was going to use to push the oil through with are veterinary syringes without the luer lock, they are just bare nipples.
I know the oil should be hot to aid filtering speed.
So my question is, can I rupture the membrane if I push the plunger too hard or am I worried about nothing?
Second question, is using veterinary syringes to push the oil through the filter a bad idea because it doesn't have to lock part of the luer lock? Will I end up creating so much pressure that I push the filter off of the nipple? (The only syringes I have that have the locking ring that the filter can screw into are 3cc, but I have probably over 300.)
Basically all my concerns are around how much pressure is going to be required to filter the finished oil.
Am I worrying over nothing? It wouldn't be the first time.
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02-16-2019, 03:55 AM #38
Those filters aren't made to filter oil. So you'll never be able to push 100ml of oil through those filters.
Oil should NEVER be hot. I never go above 40 degrees Celsius.
Yes you can rupture the membrane or crack the actual syringe filter if too much pressure is applied and/or the oil is hot.
My advice is to use luerlock syringes.am06 liked this post
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07-18-2023, 08:21 AM #39
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What media botttle and bottle top filter should k buy for a test C and prop? I can’t use syringe filters, had success with bottles tops like almost 20 years ago but then had less the next few times and was getting clogged.
The stuff with nebulizers etc are over my head with out out a a video and don’t want to spend hours melting plastic. What are my options, I. Plan to use only bb and ba and which ever oil. Not sure I like EO melting stuff and then going in my body with or with out whatever it melted. Dropped some on my computer years ago and it ate the paint right off it.
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08-05-2023, 11:14 AM #40
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