1. #1
    MuscleChemistry Newbie Residency Training

    Join Date
    Oct 2016
    Location
    Hong Kong
    Posts
    8
    Rep Power
    0

    Default How to choose right syringe filters and filter membranes

    Hi guys,


    The texture of syringe filters include Nylon, PVDF, PTFE, MCE, CA, Glass Fiber, PP, PES.


    When choosing the texture of syringe filters, we need to consider pore diameter, chemical resistance( if can resist all carrier oils and solvents), oil-based or water-based solution, and etc.


    Here are the pore diameter of common syringe filters.


    Pore diameter:
    Nylon, PVDF, PTFE, MCE, CA, PP, PES: 0.22um, 0.45um
    Glass Fiber: 0.7um, 1.2um


    Here are some common questions we enounter when choosing proper syringe filters?
    1. Is it necessary to have a 0.22 um to have a sterile solution or the 0.45 um gives acceptable results concerning the dangers of bacteria ?
    2. What is the average filtering time per 100ml oil-based injections and water-based injections? What carrier oil you use?
    3. Warming your injection will make filtering much easier. What is the filtering time difference between warmed and unwarmed oil?
    4. Which membrane are the best for a steroid based solution? Oil-based and water-based.


    Here is the chemical resistance chart of Whatman syringe filters/filter membranes.


    Nylon PVDF PTFE MCE CA Glass Fiber PP PES
    Benzyl Alcohol LR R R R LR R R NR




    R=Resistant
    LR=Limited Resistance
    NR=Not Resistant




    I would like to answer the above questions with my shallow expertise.
    1. 0.22 um is used for sterilizing-grade filtration. Literally speaking, getting rid of bacterials. 0.45um is used for clarification filtration and pre-filtration. Always stick to
    0.22 um though more time-consuming. I want to correct my words in the past posts.
    4. Just considering the pore diameter, Glass Fiber is out of the game. Then comes to chemical resistance, Nylone, CA, and PES are out of the game too. So the rest
    options might be good for use: PVDF, PTFE, MCE, PP. But deeper consideration is: would these syringe filters not be eaten by other solvents(like BB, EO, guaiacol,
    PS80), even carrier oils? As far as I know, sesame oil eats PES.


    My provisional conclusion is: 0.22 um of PVDF, PTFE, MCE, PP are good for use. But it is a open proposition.


    All MC bros are welcome to anwser these questions. Always glad to discuss this with all you guys.




    PS:
    Nylon: Nylon
    PVDF: Polyvinylidene Fluoride
    PTFE: Polyfluortetraethylene
    MCE: Mixed Cellulose Ester
    CA: Acetate Cellulose
    Glass Fiber: Glass Fiber
    PP: Polypropylene
    PES: Polyether Sulfone
    Thanks BERSERKER973, pgb thanked for this post
    Likes pgb liked this post
     

  2. #2
    MuscleChemistry Newbie Residency Training

    Join Date
    Oct 2016
    Location
    Hong Kong
    Posts
    8
    Rep Power
    0

    Default

    Whoops. @presser. Please correct these mistakes for me. I can not reedit my post here.
    1. How to chosee right syringe filters and filter membranes-- How to choose right syringe filters and filter membranes
    2. Here are the pore diameter of common syringe filters.--Here is the pore diameter of common syringe filters.
    3. 0.22 um of PVDF, PTFE, MCE, PP are good for use--0.22 um PVDF, PTFE, MCE, PP are good for use
     

  3. #3
    MuscleChemistry Vet Board Certified Psy.D

    Join Date
    Apr 2016
    Posts
    358
    Rep Power
    2147484013

    Default

    hmm, I literally just bought a few more nylon filters because I seemed to have read all over the web that was preferred.

    I didn't quite understand the way you worded it. You said nylon and pes are out because of chemical resistance. What does that mean? That solvents will eat the filter?
    Likes Chocolate Rain liked this post
     

  4. #4
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    Thanks big Iron up for this thread and concerning my questions. So .22um PVDF and ptfe ate good, correct?
    And solvents ba and bb with GSO what types of plastic or bottle tops that are resistant?
    And if using glass media bottles can they be bought sterile that way I can transfer into sealed sterile vails with syringe.
    I have brewed before but it been a while and used whatman .22 syringe filter into 50 ml veils. I used 60 ml syringe with calk gun and switch back and forth to Springs to hold pressure. Don't remember type of whatman I used I had followed a guide each time for everything.
    I have vacuum electric and hand pump don't know if I should try bottle topm
     

  5. #5
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    Quote Originally Posted by trekrider215 View Post
    hmm, I literally just bought a few more nylon filters because I seemed to have read all over the web that was preferred.

    I didn't quite understand the way you worded it. You said nylon and pes are out because of chemical resistance. What does that mean? That solvents will eat the filter?
    Correct I have read that PES definitely will melt and nylon from what I have read has to be only the ones that are chemical resistant they will bust easy and there is a plastic piece inside membrane that will melt. I read only certain types can handle heat and solvents like ba and bb. Like some of the color codes or for specific types of cultures and resistance. That is what I read.
    Better to go with pdfv is what I have read.
    Likes Chocolate Rain, PezBolic liked this post
     

  6. #6
    MuscleChemistry Vet Board Certified Psy.D

    Join Date
    Apr 2016
    Posts
    358
    Rep Power
    2147484013

    Default

    The nylon zapcap says its chemical resistant. Isnt that chem resistant what we want? Or no?

    - - - Updated - - -

    It jusy got here the mothet fucker doesnt have threads. You gotta be shitting me. It says its for 45mm glass, but it doesnt even screw on. Wtf
     

  7. #7
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    Quote Originally Posted by trekrider215 View Post
    The nylon zapcap says its chemical resistant. Isnt that chem resistant what we want? Or no?

    - - - Updated - - -

    It jusy got here the mothet fucker doesnt have threads. You gotta be shitting me. It says its for 45mm glass, but it doesnt even screw on. Wtf
    What kind is that? Relax let a mechanic teach u lol. Get some latex gloves pull them over from bottom and wrap with electric tape. That will give u a good seal.

    - - - Updated - - -

    Also if u have a nebulizer it's easy to use it as a vacuum I can tell u how to do that too or make other electric vacs

    - - - Updated - - -

    If u have any compressor u can create suction with intake with little modifications. U need a gauge and Valve to close once max vacuum pressure is created then u kick on when necessary or go check every once and while depending on leaks.

    - - - Updated - - -

    The chemical resistance should be limited resistance as stated above (LR). Maybe go with less bb and ba. Go with minimal amount necessary. If u doing enathate there are different recipes
    Thanks big_iron_up thanked for this post
    Likes big_iron_up liked this post
     

  8. #8
    MuscleChemistry Vet Board Certified Psy.D

    Join Date
    Apr 2016
    Posts
    358
    Rep Power
    2147484013

    Default

    Quote Originally Posted by Muscle mechanic View Post
    What kind is that? Relax let a mechanic teach u lol. Get some latex gloves pull them over from bottom and wrap with electric tape. That will give u a good seal.

    - - - Updated - - -

    Also if u have a nebulizer it's easy to use it as a vacuum I can tell u how to do that too or make other electric vacs

    - - - Updated - - -

    If u have any compressor u can create suction with intake with little modifications. U need a gauge and Valve to close once max vacuum pressure is created then u kick on when necessary or go check every once and while depending on leaks.

    - - - Updated - - -

    The chemical resistance should be limited resistance as stated above (LR). Maybe go with less bb and ba. Go with minimal amount necessary. If u doing enathate there are different recipes

    ah okay I see. thank you
     

  9. #9
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    U can get the sterile glove packs from pharmacy that way nothing can get into your mix. Sterile glove will cover surface area and where your top section is pull insert section of glove over that way tape touches nothing but glove. Remove with a razor knife.

    For a vacuum pump nebulizer take intake plug out and remove filter. Find a hose to fit snug inside install a push on T. Then add section of hose to a vacuum gage. Next put a valve from T. Make sure gauge is between valve and bottle top.
    To make a good seal u can find an o-ring to fit over hose and inside intake on nebulizer find o-ring thickness and diameter to give a snug fit on both hose and intake. When u ready open valve when u get to desired vac pressure shut off nebulizer and close valve. Watch gauge for next time to kick on. Or check now and then. If u make a good seal it will hold vacuum until no fluids are on top.

    All modification parts can be purchased at a auto parts store. Gauge, valve, hose, o-rings, all of it.

    Calk gun method with filter syringe is way I used to go. I have extra springs to put between for constant pressure. Make a stand to hold guns just above bottle 50 ml with filter and 60 ml syringe. I think it's 43 ml u can hold at time in syringe to fit gun. This way works and u don't have to but crank a little when tension runs out.

    I am a mechanic and very mechanically minded and inclined. I hope this helps. Will do a thread on it all later when I have time.
     

  10. #10
    MuscleChemistry Newbie Residency Training

    Join Date
    Oct 2016
    Location
    Hong Kong
    Posts
    8
    Rep Power
    0

    Default

    @trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...15100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um PVDF, PTFE, MCE, PP are good for use.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass bottle tops is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.

    - - - Updated - - -

    @trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...1015100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um of PVDF, PTFE, MCE, PP are good for us.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass units is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.
    Thanks Muscle mechanic thanked for this post
    Likes Muscle mechanic liked this post
     

  11. #11
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    Quote Originally Posted by big_iron_up View Post
    @trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...15100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um PVDF, PTFE, MCE, PP are good for use.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass bottle tops is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.

    - - - Updated - - -

    @trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...1015100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um of PVDF, PTFE, MCE, PP are good for us.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass units is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.
    Thanks for all the info. I know what to order now in place of what's not been in Stock. Will order in just a minute. I will use PVDF syringe ether Millipore or whatman. Will use calk gun method hands aching bad. Carpal tunnel need surgery.
     

  12. #12
    MuscleChemistry Newbie Residency Training

    Join Date
    Oct 2016
    Location
    Hong Kong
    Posts
    8
    Rep Power
    0

    Default

    @trekrider215


    Here is the link of Whatman chemical compatibility chart. Please check this out:
    https://www.gelifesciences.com/gehcls...1015100012.pdf


    LR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration.


    NR means that PES is Not Resistant to BA. BA wil melt PES for sure.






    @Muscle mechanic
    Yes. 0.22 um PVDF, PTFE, MCE, PP are good for us.
    For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass units is also good for use.


    Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.
    Thanks Muscle mechanic thanked for this post
    Likes Muscle mechanic liked this post
     

  13. #13
    MuscleChemistry Vet Board Certified Psy.D

    Join Date
    Apr 2016
    Posts
    358
    Rep Power
    2147484013

    Default

    Quote Originally Posted by big_iron_up View Post
    @trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...15100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um PVDF, PTFE, MCE, PP are good for use.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass bottle tops is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.

    - - - Updated - - -

    @trekrider215Here is the link of Whatman chemical compatibility chart. Please check this out:https://www.gelifesciences.com/gehcls...1015100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um of PVDF, PTFE, MCE, PP are good for us.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass units is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.
    Okay, thank you this was an excellent post. Cleared things up a lot for me.

    Well, i'm running 100ml's through a nylon .22 Nalgene with glass media bottle because it was the only thing I bought because I was initially under the impression nylon was the way to go. So far it's going very well. just kind of slow. I'm sure your right that there is a cut off point in which it begins to melt the filter, this might explain my first batch.

    @musclemechanic, I truly appreciate you guidance on how to rig my filter up. I tried to make a seal with a glove and duct tape but it still didn't seem to hold. I just don't understand how they can make a bottletop filter and say it's for a 45mm glass media bottle and not put fucking threads on the bottom of it. I'm calling medlabsupplys and making them send me a replacement. That's bullshit. I just don't understand how its supposed to hold a vacuum without a tight seal. I shouldn't have to rig it up. It's only a 12 dollar tool so no big deal, i'm sure they'll send a replacement. For the record this is the .22 Nylon Whataman Zapcap 500ml bottle top filter. If anyone reads this, don't buy one!

    The Nalgene seems to be getting it done though. A few pumps of the hand vac and it's on its way. The glass medium bottle made a huge difference from the other night when I first tried because I can get much more suction with the glass media bottle and not worry about it cracking.

    - - - Updated - - -



    - - - Updated - - -



    - - - Updated - - -

    My bad, I didn't mean to post the picture twice. It won't let me delete it. Anyways, you can see what a big difference it made. I have no idea why the gear up top looks like it came out of someones rotten kidneys lol.
    Thanks Muscle mechanic thanked for this post
    Likes Muscle mechanic liked this post
     

  14. #14
    MuscleChemistry Vet Board Certified Psy.D

    Join Date
    Apr 2016
    Posts
    358
    Rep Power
    2147484013

    Default

    So after 6 hours lol, i was able to get about 90mls through the filter pictured up above. It got to a point where it literally just wouldnt drip at all and im pretty sure i may have even maxed the pressure out on the hand pump.
    The filtered media came out literlly flawless, crystal clear. Theres still about 30-40 unfiltered i poured into a jar and will syringe filter tommorow.

    Im a little suprised i couldnt get the full amount through it. I did notice obvious signs that the top cup was being eaten by the solvents, so i can only assume that somehow the internal working were damaged causing it to some how clog up. This may be the point big iron talked about.

    I see no signs that any of it actually ended up in the geqr though. Oil is crystal clear.

    Filter head pressure to the very end unlike the other filts i tried that i couldnt get it to hold for even a few minutes.

    Had i got the bigger version of this filter i could have had twice the diameter of filter which i imagine woul have greatly increased the speed.

    Pvdf will be my next order to compare. I think nylon and pes can be used you just have to pay attention. Its obvious this stuff chews up the plastic pretty well.

    The first batch i made i did exactly as i did tonight with sane filter and everything and i now know the reason it cane out cloudy was because of the plastic media bottle i used then versus the glass media bottle I used tonight.

    - - - Updated - - -

    You can actually see in the pic above. The top bottle cop, the hazy line above the oil. Thats not oil. That foggy area is the plastic being basically dissolved.
     

  15. #15
    Presser's Avatar
    Join Date
    May 2002
    Posts
    41,686
    Rep Power
    2147525355

    Default

    ok, I just went to the link you posted to see what the hell y'all were talking about, and who the fuck understands this shit lmao, Fuck man you need a Chemistry Degree to read that shit
    Likes pgb liked this post
     
    How to choose right syringe filters and filter membranes

  16. #16
    MuscleChemistry Newbie Residency Training

    Join Date
    Oct 2016
    Location
    Hong Kong
    Posts
    8
    Rep Power
    0

    Default

    Well, I have to say that comprehending this thread is like cracking nutshells. Once cracked, you find it nutritious.
    Likes Presser, pgb liked this post
     

  17. #17
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    Ptfe. Seams best have looked more last night on Millipore says nylon and another site syringe filters say ptfe for bb.
    They just say to use a prolypropylene housing and top going into glass. So brand may have alot to do with it. I will email a manufacturer and ask recommended type for bb and ba
    Likes Presser liked this post
     

  18. #18
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    @trekrider, so I am gathering that the filter filters any plastic residue that melts from top section. So even prolypropylene will melt or be eaten up some?
    Be specified of filter used and top with media bottle please. Brand, type, size, housing type(prolypropylene?), Pore size.
    Your zapcap is supposed to create a seal on any media bottle standard size. That's odd that it wouldn't. It is compatible just as luer lock and luer slip. Talked to a buddy this morning who told me that's how zapcap seals and works. I never used just saying what he said.

    I play dumb to anyone I know I just tell them someone asked on forum. I have never told a soul that I have brewed before or plan on doing it. People just talk too much shit! Lol
     

  19. #19
    MuscleChemistry Newbie Residency Training

    Join Date
    Dec 2016
    Posts
    2
    Rep Power
    0

    Default

    Hey bro Maine Manufacturing makes the zapcaps now and I still love 'em. They are tougher than the Millipore steritops or cups [I had at least 5 leak on me and I've been doing this almost 15 yrs].
    Nylon is your best all purpose. If you have 100% EO or want to filter someting like Guiacol so it's 'sterile' you'll want the 'PTFE' membrane (It's similar/partially teflon).
    One gripe I get is that other than the cellulose acetate (just say no) zaps are NOT sterile. Easy fix. Have a bottle of 0.22 filtered BA and spray it on the filter surface. Attach vacuum source. Drip,drip,drip... NOW it's sterile ! Use a 0.45 micron first. NEVER, EVER be a tightass and start with a 0.22. You'll eat up filters and waste money. Ideally filtering thru a 0.8 micron and 0.45 micron nylon filter sheets (vacuum flask setup) is best but it takes looking.
    Here's a trick. Have some textured gauze. When the flow slows down I suck the liquid out into glassware. I then clean the surface well and microwave the liquid to a reasonable (consult your reference temp for membrane). You will get about 750ml out of 500 -or should unless you are getting powders far downstream). Same goes for the 0.22 Nylon zapcap- I recommend a basic MCT 60/40 (c8/c10) mix. Nobody is getting 500 without pre-filtering. MAYBE with Digycol (840?) which has a viscosity of 10. Grapeseed oil is 54, CSO is 76.
     

  20. #20
    MuscleChemistry Newbie Residency Training

    Join Date
    Dec 2016
    Posts
    2
    Rep Power
    0

    Default

    Hey bro Maine Manufacturing makes the zapcaps now and I still love 'em. They are tougher than the Millipore steritops or cups [I had at least 5 leak on me and I've been doing this almost 15 yrs].
    Nylon is your best all purpose. If you have 100% EO or want to filter someting like Guiacol so it's 'sterile' you'll want the 'PTFE' membrane (It's similar/partially teflon).
    One gripe I get is that other than the cellulose acetate (just say no) zaps are NOT sterile. Easy fix. Have a bottle of 0.22 filtered BA and spray it on the filter surface. Attach vacuum source. Drip,drip,drip... NOW it's sterile ! Use a 0.45 micron first. NEVER, EVER be a tightass and start with a 0.22. You'll eat up filters and waste money. Ideally filtering thru a 0.8 micron and 0.45 micron nylon filter sheets (vacuum flask setup) is best but it takes looking.
    Here's a trick. Have some textured gauze. When the flow slows down I suck the liquid out into glassware. I then clean the surface well and microwave the liquid to a reasonable (consult your reference temp for membrane). You will get about 750ml out of 500 -or should unless you are getting powders far downstream). Same goes for the 0.22 Nylon zapcap- I recommend a basic MCT 60/40 (c8/c10) mix. Nobody is getting 500 without pre-filtering. MAYBE with Digycol (840?) which has a viscosity of 10. Grapeseed oil is 54, CSO is 76.
     

  21. #21
    MuscleChemistry Newbie Residency Training

    Join Date
    Dec 2016
    Posts
    2
    Rep Power
    0

    Default

    Quote Originally Posted by big_iron_up View Post
    Hi guys,


    The texture of syringe filters include Nylon, PVDF, PTFE, MCE, CA, Glass Fiber, PP, PES.


    When choosing the texture of syringe filters, we need to consider pore diameter, chemical resistance( if can resist all carrier oils and solvents), oil-based or water-based solution, and etc.


    Here are the pore diameter of common syringe filters.


    Pore diameter:
    Nylon, PVDF, PTFE, MCE, CA, PP, PES: 0.22um, 0.45um
    Glass Fiber: 0.7um, 1.2um


    Here are some common questions we enounter when choosing proper syringe filters?
    1. Is it necessary to have a 0.22 um to have a sterile solution or the 0.45 um gives acceptable results concerning the dangers of bacteria ?
    2. What is the average filtering time per 100ml oil-based injections and water-based injections? What carrier oil you use?
    3. Warming your injection will make filtering much easier. What is the filtering time difference between warmed and unwarmed oil?
    4. Which membrane are the best for a steroid based solution? Oil-based and water-based.


    Here is the chemical resistance chart of Whatman syringe filters/filter membranes.


    Nylon PVDF PTFE MCE CA Glass Fiber PP PES
    Benzyl Alcohol LR R R R LR R R NR




    R=Resistant
    LR=Limited Resistance
    NR=Not Resistant




    I would like to answer the above questions with my shallow expertise.
    1. 0.22 um is used for sterilizing-grade filtration. Literally speaking, getting rid of bacterials. 0.45um is used for clarification filtration and pre-filtration. Always stick to
    0.22 um though more time-consuming. I want to correct my words in the past posts.
    4. Just considering the pore diameter, Glass Fiber is out of the game. Then comes to chemical resistance, Nylone, CA, and PES are out of the game too. So the rest
    options might be good for use: PVDF, PTFE, MCE, PP. But deeper consideration is: would these syringe filters not be eaten by other solvents(like BB, EO, guaiacol,
    PS80), even carrier oils? As far as I know, sesame oil eats PES.


    My provisional conclusion is: 0.22 um of PVDF, PTFE, MCE, PP are good for use. But it is a open proposition.


    All MC bros are welcome to anwser these questions. Always glad to discuss this with all you guys.




    PS:
    Nylon: Nylon
    PVDF: Polyvinylidene Fluoride
    PTFE: Polyfluortetraethylene
    MCE: Mixed Cellulose Ester
    CA: Acetate Cellulose
    Glass Fiber: Glass Fiber
    PP: Polypropylene
    PES: Polyether Sulfone
    Believe it or not the Foxx 0.45 PES can take 250mg (1% BA, 10% BB) enanthate in GSO or MCT oil like a it's a big gulp. I wouldn't rely on 0.22 but you'd probably be OK with it. I am not sure why nylon isn't rated higher. For chemikaze's using Guiacol or other potent solvents I used to say PVDF but Millipores bottle unit's IMO have been shitty whereas I have beaten the living dogshit out of Zapcaps and they overperform by 50%+ .
    PTFE filters strong acids and & aldehydes.
    Likes Muscle mechanic, big_iron_up liked this post
     

  22. #22
    MuscleChemistry Newbie Board Certified Psy.D

    Join Date
    Nov 2013
    Posts
    56
    Rep Power
    109

    Default

    The Millipore ones I have used int he past have all off sudden started clogging and its super hard to keep pumping
     

  23. #23
    MuscleChemistry Newbie Board Certified D.V.M.

    Join Date
    Apr 2014
    Posts
    34
    Rep Power
    0

    Default

    I use whatmam zap cap CR and nothing else best filters ever. They don't screw on but work great. Use a 45 neck glass media bottle.

    Sent from my LG-H830 using Tapatalk
     

  24. #24
    MuscleChemistry Newbie Board Certified D.V.M.
    Tailor's Avatar
    Join Date
    Mar 2017
    Posts
    30
    Rep Power
    0

    Default

    zapcap cr coming in 0.45um. Is it enough for proper filtration?
     

  25. #25
    MuscleChemistry Registered Member Board Certified MD
    Muscle mechanic's Avatar
    Join Date
    Sep 2016
    Posts
    3,675
    Rep Power
    2147487330

    Default

    Quote Originally Posted by Tailor View Post
    zapcap cr coming in 0.45um. Is it enough for proper filtration?
    .45 is considered sterile.
    And I am pretty sure most labs use It!
    Anyway they say certain virus's can get thru but u don't get I viral abscess only bacteria ones!
    Ba will stop production of viruses and they will not be preserved in your vial to become active. They need a host or they die!
    But still baking is done with. 45 and the time saved u can bake a cake and lots of stuff while oven us hot!

    Sent from my SAMSUNG-SM-G890A using Tapatalk
    Thanks Tailor thanked for this post
     
    Just a grateful member!!

  26. #26
    MuscleChemistry Senior Board Certified Psy.D
    chihuahua's Avatar
    Join Date
    Jan 2017
    Location
    Lone Star State
    Posts
    400
    Rep Power
    2147484055

    Default

    .45 PVDF sterile Whatman.. Done. If you want to be there all day by all means use the .22. I have yet to come across anyone that has gotten an infection from using a .45. Maybe I'm just lucky when comes to filtering. Good luck
    Thanks Muscle mechanic, Tailor thanked for this post
    Likes Muscle mechanic liked this post
     

  27. #27
    MuscleChemistry Newbie Residency Training

    Join Date
    Aug 2017
    Posts
    20
    Rep Power
    0

    Default

    Quote Originally Posted by trekrider215 View Post
    hmm, I literally just bought a few more nylon filters because I seemed to have read all over the web that was preferred.

    I didn't quite understand the way you worded it. You said nylon and pes are out because of chemical resistance. What does that mean? That solvents will eat the filter?
    These links are compatibilty charts. There are some things missing but you will get an idea. PES is only ok for a prefilter of a very low BA/BB mix. Nylon is the best overall but prefiltering ie a 0.8 or 1.0 micron nylon syringe filter or 47mm membrane (all found in nylon) are ideal if you want to save your filters. I am going to put a post dispel a lot of myths are things people are doing wrong. How to choose right syringe filters and filter membranes-1494091598515.jpgHow to choose right syringe filters and filter membranes-10mlvetsyring.jpg
    https://sevierlab.vet.cornell.edu/res...art-Detail.pdf
    https://www.treborintl.com/content/ch...sistance-chart

    Pics (right) Millipore 0.22 micron PVDF STERIVEX. Prefiltered and using the veterinary syringe NOBODY SHOULD OR NEED TO USE A CAULK GUN. That syringe is $8 at any tractor supply or online. Guys, You've gotta prefilter. It is easier and saves you money. You can't be a cheapskate.
    Attached Thumbnails Attached Thumbnails How to choose right syringe filters and filter membranes-10mlvetsyring.jpg   How to choose right syringe filters and filter membranes-1494091598515.jpg  
     

  28. #28
    MuscleChemistry Newbie Residency Training

    Join Date
    Aug 2017
    Posts
    20
    Rep Power
    0

    Default

    One thing about this thread. I am fairly certain the ratings against Benzyl Alcohol,BB, Acetone,ect is if the ENTIRE sample is that chemical. I can see this from empirical experience. Nylon needs to be 'nylon 66' made material. It works. However, if one were filtering pure solvents PTFE is the only way to go. EO is falling way out of favor as far as I can tell and guiacol works but it stinks and it comes thru skin pores. Whomever is content with using 0.45 micron pore size remember staph aureus can routinely get thru this size. The link below even says 0.22 membranes can have this happen. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896367/
    Oil and BA help a lot. Water is like a petri dish. Theoretically 0.1 micron filters would be as good as one could get but that is not practical. You have to be a search engine magician to find them in a syringe filter of a suitable membrane.
     

  29. #29
    MuscleChemistry Newbie Residency Training

    Join Date
    May 2017
    Posts
    9
    Rep Power
    0

    Default

    Quick question

    Can someone explain the differences between inlet outlet female male?

    Getting some filters and they have female inlet and male outlet. Are these OK to use? I have PDVF 0.22um 33mm and PES 0.10um 33mm both sterile. The first used for oils and the second for amino acids in water.

    Thanks
     

  30. #30
    MuscleChemistry Newbie Residency Training

    Join Date
    Aug 2017
    Posts
    20
    Rep Power
    0

    Default

    Using a 90mm (or whatever diameter the membrane is) nylon or PVDF filter membrane ON top of say the 0.22 micron PES will do the dirty work and should fix this problem. Unless already pre-filtered a 0.65-0.45 micron size will do it. Just look at the drip. If it rolls through like the Wermacht thru Poland you are kind of screwed. PES ones that sell for $13 are not a good buy. Maybe if they are $5 each in a box of 12 (fire sale price) they can be useful. If someone is making a bunch of bacteriostatic water or filtering GSO, CSO, or MCT oil. It' s not a problem. Just my $0.02.
     

  31. #31
    MuscleChemistry Newbie Residency Training

    Join Date
    Aug 2017
    Posts
    20
    Rep Power
    0

    Default

    Quote Originally Posted by newwave View Post
    Quick question

    Can someone explain the differences between inlet outlet female male?

    Getting some filters and they have female inlet and male outlet. Are these OK to use? I have PDVF 0.22um 33mm and PES 0.10um 33mm both sterile. The first used for oils and the second for amino acids in water.

    Thanks
    I think that is the normal way. The syringe goes in, or screws to the 'female' top and the male has the 'dong' for lack of a better term at the end. PES should be fine for aminos. It just depends on what the preservative/anti-microbial is (parabens, ect) .
    Thanks newwave thanked for this post
    Likes newwave liked this post
     

  32. #32
    MuscleChemistry Newbie Residency Training

    Join Date
    Aug 2017
    Posts
    20
    Rep Power
    0

    Default

    Quote Originally Posted by Tailor View Post
    zapcap cr coming in 0.45um. Is it enough for proper filtration?
    Cellulose Acetate is not anywhere near ideal. If you had a 70mm membrane set on top of it that would help a lot. That's the size they come in. Hard to come across unless you look quite a bit. And never use Nalgene Nylon filters. They suck and Foxx bioscience is weak too (membrane is not very thick). At least with those they take 90mm membranes. Watch the flow rate. It should be a steady drip (quick) not like sucking Pepsi through a straw.
     

  33. #33
    MuscleChemistry Newbie Residency Training

    Join Date
    Aug 2017
    Posts
    20
    Rep Power
    0

    Default

    Quote Originally Posted by big_iron_up View Post
    Hi guys,


    The texture of syringe filters include Nylon, PVDF, PTFE, MCE, CA, Glass Fiber, PP, PES.


    When choosing the texture of syringe filters, we need to consider pore diameter, chemical resistance( if can resist all carrier oils and solvents), oil-based or water-based solution, and etc.


    Here are the pore diameter of common syringe filters.


    Pore diameter:
    Nylon, PVDF, PTFE, MCE, CA, PP, PES: 0.22um, 0.45um
    Glass Fiber: 0.7um, 1.2um


    Here are some common questions we enounter when choosing proper syringe filters?
    1. Is it necessary to have a 0.22 um to have a sterile solution or the 0.45 um gives acceptable results concerning the dangers of bacteria ?
    2. What is the average filtering time per 100ml oil-based injections and water-based injections? What carrier oil you use?
    3. Warming your injection will make filtering much easier. What is the filtering time difference between warmed and unwarmed oil?
    4. Which membrane are the best for a steroid based solution? Oil-based and water-based.


    Here is the chemical resistance chart of Whatman syringe filters/filter membranes.


    Nylon PVDF PTFE MCE CA Glass Fiber PP PES
    Benzyl Alcohol LR R R R LR R R NR




    R=Resistant
    LR=Limited Resistance
    NR=Not Resistant




    I would like to answer the above questions with my shallow expertise.
    1. 0.22 um is used for sterilizing-grade filtration. Literally speaking, getting rid of bacterials. 0.45um is used for clarification filtration and pre-filtration. Always stick to
    0.22 um though more time-consuming. I want to correct my words in the past posts.
    4. Just considering the pore diameter, Glass Fiber is out of the game. Then comes to chemical resistance, Nylone, CA, and PES are out of the game too. So the rest
    options might be good for use: PVDF, PTFE, MCE, PP. But deeper consideration is: would these syringe filters not be eaten by other solvents(like BB, EO, guaiacol,
    PS80), even carrier oils? As far as I know, sesame oil eats PES.


    My provisional conclusion is: 0.22 um of PVDF, PTFE, MCE, PP are good for use. But it is a open proposition.







    PS:
    Nylon: Nylon
    PVDF: Polyvinylidene Fluoride
    PTFE: Polyfluortetraethylene
    MCE: Mixed Cellulose Ester
    CA: Acetate Cellulose
    Glass Fiber: Glass Fiber
    PP: Polypropylene
    PES: Polyether Sulfone

    The context needs to be made. Resistance is relative. Keep in mind this is referencing SRTAIGHT BA, BB, ect. so even the wimpy PES can handle some things in small amounts but I would avoid altogether. Nylon: It gets a bad rap. It's a good membrane as long as them membrane is adequately thick
    .
    PDFE - there are hydroPHILIC and hydroPHOBIC versions- that being said this is for filtering PURE SOLVENTS. You can use it but I can tell you this much, use it in syringe filters and get out a fucking sundial.

    PVDF: All purpose, hydrophilic, and is good. Nylon is cheaper , and for the most part is perfectly ok. For a syringe use PVDF gets the nod.

    MCE: I'm not sold on it. Have no feedback.
    AS ALWAYS DO RESEARCH AND PREFILTER. DON'T SPEND $6.00 TO FILTER 30CC OR RUIN YOUR THUMBS. I have no idea why I see dudes wasting $$ using 0.22 only. YES-use them, but find a larger pore and you will have a much easier time. Spend a little to save a lot of money and effort. Hint -ebay.
     

  34. #34
    MuscleChemistry Newbie Residency Training

    Join Date
    Aug 2017
    Posts
    20
    Rep Power
    0

    Default

    Sorry about the gigantic font. 47mm filters and the 300ml beaker top with metal clamps and receiver cost maybe $50. Find some and if you want sterile then use the syringe filters. For the cost of a 1.5 cfm 1/4 HP pump ($90 @ Harbor Freight) you are set. MCT oil is 1/2 the viscosity of GSO and by the gallon it's $40. I dont know who would need that much so start cooking with it and putting it in shakes.
     

  35. #35
    MuscleChemistry Newbie Board Certified Psy.D

    Join Date
    Jan 2016
    Posts
    59
    Rep Power
    179

    Default

    Quote Originally Posted by trekrider215 View Post
    The nylon zapcap says its chemical resistant. Isnt that chem resistant what we want? Or no?

    - - - Updated - - -

    It jusy got here the mothet fucker doesnt have threads. You gotta be shitting me. It says its for 45mm glass, but it doesnt even screw on. Wtf
    Those seal too the media bottle with vacuum once you pull a vacuum it will suction too the top of the media bottle bro.

    Sent from my LG-TP450 using Tapatalk
     

  36. #36
    MuscleChemistry Newbie Board Certified D.V.M.

    Join Date
    Mar 2017
    Posts
    46
    Rep Power
    0

    Default

    What is an inorganic filter membrane at .22 microns what type it's it?
    nouler Juler 1000Ml Filter Unit Vacuum Unit + 50X 0.22Um Inorganic Filter Membrane for Filtration of Aqueous Solution Amazon.com: nouler Juler 1000Ml Filter Unit Vacuum Unit + 50X 0.22Um Inorganic Filter Membrane for Filtration of Aqueous Solution: Sports & Outdoors

    Sent from my S60 using Tapatalk
     

  37. #37
    MuscleChemistry Newbie Board Certified Psy.D

    Join Date
    Nov 2018
    Location
    Most likely at the gym
    Posts
    71
    Rep Power
    183

    Default

    I am brand new to home brew. I have finally gotten all of the things I think I need. (I had to wait a good bit to get the glass rods for stirring)

    I have syringe filters. They are supposed to be good up to 100mls, they are the 25mm size.

    After continually reading I am concerned. Can I actually end up rupturing the membrane in the filter if I try to push it through with to much force? They are PVDF filters. The "large" volume syringes I was going to use to push the oil through with are veterinary syringes without the luer lock, they are just bare nipples.

    I know the oil should be hot to aid filtering speed.

    So my question is, can I rupture the membrane if I push the plunger too hard or am I worried about nothing?

    Second question, is using veterinary syringes to push the oil through the filter a bad idea because it doesn't have to lock part of the luer lock? Will I end up creating so much pressure that I push the filter off of the nipple? (The only syringes I have that have the locking ring that the filter can screw into are 3cc, but I have probably over 300.)

    Basically all my concerns are around how much pressure is going to be required to filter the finished oil.
    Am I worrying over nothing? It wouldn't be the first time.
     

  38. #38
    MuscleChemistry Vet Board Certified Psy.D
    thx's Avatar
    Join Date
    Jul 2015
    Posts
    387
    Rep Power
    2147484043

    Default

    Quote Originally Posted by 1_whocares View Post
    I am brand new to home brew. I have finally gotten all of the things I think I need. (I had to wait a good bit to get the glass rods for stirring)

    I have syringe filters. They are supposed to be good up to 100mls, they are the 25mm size.

    After continually reading I am concerned. Can I actually end up rupturing the membrane in the filter if I try to push it through with to much force? They are PVDF filters. The "large" volume syringes I was going to use to push the oil through with are veterinary syringes without the luer lock, they are just bare nipples.

    I know the oil should be hot to aid filtering speed.

    So my question is, can I rupture the membrane if I push the plunger too hard or am I worried about nothing?

    Second question, is using veterinary syringes to push the oil through the filter a bad idea because it doesn't have to lock part of the luer lock? Will I end up creating so much pressure that I push the filter off of the nipple? (The only syringes I have that have the locking ring that the filter can screw into are 3cc, but I have probably over 300.)

    Basically all my concerns are around how much pressure is going to be required to filter the finished oil.
    Am I worrying over nothing? It wouldn't be the first time.
    Those filters aren't made to filter oil. So you'll never be able to push 100ml of oil through those filters.

    Oil should NEVER be hot. I never go above 40 degrees Celsius.

    Yes you can rupture the membrane or crack the actual syringe filter if too much pressure is applied and/or the oil is hot.

    My advice is to use luerlock syringes.
    Likes am06 liked this post
     
    Sink, suffer, self-destruct. Rise stronger, reconstruct

  39. #39
    MuscleChemistry Newbie Board Certified Psy.D

    Join Date
    Nov 2013
    Posts
    56
    Rep Power
    109

    Default

    What media botttle and bottle top filter should k buy for a test C and prop? I can’t use syringe filters, had success with bottles tops like almost 20 years ago but then had less the next few times and was getting clogged.

    The stuff with nebulizers etc are over my head with out out a a video and don’t want to spend hours melting plastic. What are my options, I. Plan to use only bb and ba and which ever oil. Not sure I like EO melting stuff and then going in my body with or with out whatever it melted. Dropped some on my computer years ago and it ate the paint right off it.
    Thanks Presser thanked for this post
    Likes Presser liked this post
     

  40. #40
    Presser's Avatar
    Join Date
    May 2002
    Posts
    41,686
    Rep Power
    2147525355

    Default

    Quote Originally Posted by BkMothaFuka View Post
    What media botttle and bottle top filter should k buy for a test C and prop? I can’t use syringe filters, had success with bottles tops like almost 20 years ago but then had less the next few times and was getting clogged.

    The stuff with nebulizers etc are over my head with out out a a video and don’t want to spend hours melting plastic. What are my options, I. Plan to use only bb and ba and which ever oil. Not sure I like EO melting stuff and then going in my body with or with out whatever it melted. Dropped some on my computer years ago and it ate the paint right off it.

    as long as you heat the carrier oil to 250 degrees and use ba and bb, you should be fine without the filter . USP grade oil like grape or mct works best
    How to choose right syringe filters and filter membranes

Similar Threads

  1. Replies: 3
    Last Post: 10-27-2016, 11:52 PM
  2. Syringe Filters
    By FlexP in forum Steroid Powder Recipe Discussion
    Replies: 10
    Last Post: 08-11-2015, 10:25 AM
  3. Syringe filter
    By yossi1711 in forum Steroid Powder Recipe Discussion
    Replies: 5
    Last Post: 03-21-2014, 02:33 PM
  4. syringe filters
    By Carlito in forum Steroid Powder Recipe Discussion
    Replies: 2
    Last Post: 07-03-2005, 10:07 AM

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  

Log in

Log in