Stericup filters

Hey bro's those of you familiar with the high volume stericup vacuum filters. Do you need a vacuum hand pump with those or can you use them with out a hand pump?

I tried it out with a hand pump and you might be lucky to get one drop a minute out of that peice of shit.

I have used the 150ml stericup filters with a hand vacuum pump and filtered 50cc in 8 minutes with a .45um membrane.

do you think its a big difference from filterng by individual filters? You still gotta apply pressure. It might save you some arm work but very little in my opinion. Where did you get the hand pump and membranes? My hand pump fukin sucked. I got it off ebay.

For me it made a huge difference. With a regular whattman syringe filter you are applying constant pressure with your fingers. With the stericup filters and vacuum pump I pumped it up about 6 times or so and it does all the work by itself. I found that you only needed to then give it an additional pump or two about every 3 minutes or so. I thought it took all the work out of it personally. I would not recommend using it for jobs under 50 ml though.

I got my hand pump here.
http://www.cynmar.com/product_info....=4414&osCsid=64581593642e209f560d4428a8dbff1d

Try this place for filters:
https://www1.fishersci.com/Coupon?gid=105603&cid=1328

Thrower74, very cool, this is what im looking for

I pissed, I paid $50 for that same pump at researchlabsupply.com.
At Cynmar it's $20.

Oh yea, the vacuum pumps safe me lots of time and finger work too.

WTF?? I didnt post that shit!!...I said I DID NOT like the vacuum pump with stericup..shit came out drop by drop..I wasted a lot of money on it...wont be trying it out again for a while until I get mor epeople tp try and post exactly what brand and pump works best..

I don't post a lot here, but Brew can atest to my experience. I have used Stericups for well over a year now and really like them a lot. There are some things that will make your filtering go much better. First, I preffer the PVDF .45um. They filter much quicker. Second, the thickness and viscosity of your solution plays a big part in how quickly it takes your progect to filter. Third, the higher the consentration of hormone the slower the process goes. Lastly, the temp of the solution needs to be less then 110 degrees to prevent breakdown of the plastic. It will filter much quicker at high temps, but the vesel walls will breakdown and pass through to the final product. Let me give you my worst case experience. It was 1,000 mls of EQ, it took almost 9 hours. I have since adjusted my recipe a tad and thined it out. This was also a .22 filter. My average 1,000 ml project with a .45 filter now takes me from 60-120 minutes.

One other item of interest is to ensure there are no vacuum leaks. This can slow the process considerably. Hope this helps. Hope this helps. Take care. TY

sacrificing time for sterily is what would scare me the most. i do not skip time consuming tasks if they will keep me safe. spores of staph can pass right through the .45 as much as bacteria and molds etc. the .22-20 they cannot and it takes more time but much safer. also you will need to remember if you use the vacuum pumps dont you have to put it into something by removing the lid or something else exposing it to air again?

just a thought.

Not really looking for an argument, but your second sentence completely discredits any of your following statements. You are obviously not from the medical profession. First, staph is not a spore, it's bacteria. Second, "Staphylococci are Gram-positive cocci about 0.5 - 1.0 µm in diameter". Hmmm, sounds bigger then .45 um. If you would like to learn further please let me know. I have quite a few great sites. I would recommend the Post Graduate Medical Journal for a good place to start. If you can't find the information with the limited free archive let me know and I'll login and pull whatever you like.

For the record you are correct about exposing gear to the open atmosphere, but if it is exposed more with this type of filtering over the use of a kit someone has done something very-very wrong. TY


http://pmj.bmjjournals.com/