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muscle123ap
10-09-2005, 09:26 PM
After I filter my product through a stericup filter, I want to put it into a few 100ml vials. What I do is this:
Clean a funnel, submerge in 95% ethanol and shake as dry as possible.
Open the sterile 100ml vial and put the funnel in.
Open the bottle with the filtered product and pour it into the vial through the funnel.
After I'm done, I take a rubber stopper that has been submerged in 95% ethanol, shake it dry and put it on the 100ml vial.

Am I keeping it sterile enough? Any problem with a little bit of ethanol being left on the funnel? Any better ways of doing this?

Thanks!

brew
10-10-2005, 07:41 PM
I think your fine. If your worried about a little more alcohol then just let it evaporate. If its a glasss funnel bake it after the ethenal for a few minutes, it will evap off. Work on your pouring and you can eliminate a funnel and a potential step of contamination, I haven't used on in a while.