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Presser
03-03-2014, 10:59 AM
Insulin-Like Growth Factor-1 Increases Synthesis of Collagen Type I via Induction of the mRNA-Binding Protein LARP6 Expression and Binding to the 5' Stem-loop of Col1a1 and Col1a2 mRNA.
Blackstock C1, Higashi Y, Sukhanov S, Shai SY, Stefanovic B, Tabony AM, Yoshida T, Delafontaine P.
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AbstractCollagen content in atherosclerotic plaque is a hallmark of plaque stability. Our earlier studies showed that insulin-like growth factor-1 (IGF-1) increases collagen content in atherosclerotic plaques of Apoe-/- mice. To identify mechanisms we investigated the effect of IGF-1 on the la ribonucleoprotein domain family member 6 (LARP6). LARP6 binds a stem-loop motif in the 5'UTR of the mRNAs encoding the collagen type I alpha subunits (α1(I) and α2(I)), and coordinates their translation into the heterotrimeric collagen type I molecule. In human aortic smooth muscle cells (SMCs), IGF-1 rapidly increased LARP6 expression and the rate of collagen synthesis and extracellular accumulation. IGF-1 increased both LARP6 and collagen type I expression via a posttranscriptional and translation-dependent mechanism involving PI3K/Akt/p70S6k-signaling. Immunoprecipitation of LARP6, followed by qPCR indicated that IGF-1 increased the level of COL1a1 and COL1a2 mRNA bound to LARP6. Mutation of the 5'stem-loop of Col1a1 mRNA, which inhibits binding of LARP6, abolished the ability of IGF-1 to increase synthesis of collagen type I. Furthermore, overexpression of a 5'stem-loop RNA molecular decoy that sequesters LARP6, prevented the ability of IGF-1 to increase pro-α1(I) and mature α1(I) expression in cultured medium. IGF-1 infusion in Apoe-/- mice increased expression of LARP6 and pro-α1(I) in aortic lysates, and SMC-specific IGF-1-overexpression robustly increased collagen fibrillogenesis in atherosclerotic plaque. In conclusion, we identify LARP6 as a critical mediator by which IGF-1 augments synthesis of collagen type I in vascular smooth muscle, which may play an important role in promoting atherosclerotic plaque stability.


KEYWORDS:5'Stem-loop, Acheron, Akt, Atherosclerosis, Fibrillogenesis, Insulin-like growth factor (IGF), Protein synthesis, Translation, Vascular smoothmuscle cells, posttranscriptional

Presser
03-10-2014, 07:17 AM
bump

Presser
06-01-2014, 10:47 AM
bump for the recent question on igf, it was a tendon question, i know this is collagen article but collagen makes up tendons, but we have similar study on tendons by the same journal who did this study above, and sorry i said it was only a page or two back and i think I'm like 6 pages back lol, so its way back here someplace ill keep searching! didn't realize how fast our article pages fill up, geeez lol

Presser
06-01-2014, 10:52 AM
15425

bigdaddyo1617
06-01-2014, 11:06 AM
sweet

Presser
11-12-2014, 08:52 AM
someone was just asking about this the other day so big bump

Presser
12-26-2014, 09:26 AM
bump for another great read

Presser
01-18-2016, 11:13 AM
just another IGF-1 lr3 information bump!

kgetbig
01-21-2016, 10:09 AM
How are you doing. Presser im near philly dependent on how much and how much i can afford but you are tempting me.if i cant afford it right now.. I! Gonna have to save for it. I. Determined to get big and massive.


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Presser
01-21-2016, 10:23 AM
what does you being near philly have to do with anything

freakinthegym
01-21-2016, 04:09 PM
what does you being near philly have to do with anything

hopefully its a type 0 lol

bump for best igf around ! MC store is top notch!

Iron Game
01-22-2016, 12:43 AM
How are you doing. Presser im near philly dependent on how much and how much i can afford but you are tempting me.if i cant afford it right now.. I! Gonna have to save for it. I. Determined to get big and massive.


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MC has the best IGF. You wont regret your decision.

kgetbig
01-22-2016, 12:50 AM
I could use help in using this wiseley

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Presser
02-03-2016, 10:31 AM
someone was just asking about how to strengthen their tendons and what they could use. So here is a solid read on Collagen Synthesis by way of Lr3 IGF-1

Presser
11-23-2017, 09:54 AM
Bump

Presser
01-19-2019, 02:08 PM
Igf 1 healing through synthesis of collagen aka tendons and ligaments