Homebrew Help, Vials ALWAYS have Floaters

F

frizzleman

New member
I have been trying to get this shit right for a LONG LONG time now. INCREDIBLY frustrated. This is what I do.


Bathe Vials in Rubbing Alcohol.
Rinse with Tap Water 6 times
Put face down on pan lined with tin foil
Place in Oven at 450 for 2 hours (overkill for sterilization)

No matter how many times I rinse these fucking vials as soon as they come out of the oven and something goes into them there are white floaties. 90% of the Vials look contaminated with shit. I thought maybe my filter was shitty so I took a vial right from the oven and put poland spring water in them and the same damn thing. White floaty bullshit in the vials.

I have no idea what I am doing wrong but its getting completely exhausting. Please dont just get on here and tell me to use pre-sterilized vials... that does not help.

Does tap water somehow leave white floaty bullshit in the vials? Cant see how since I hold new vials after the Alcohol bath up to a light with tap water in them and they are completely clear with no floaters. Somehow after the oven, most vials are trash. I am at the end of my rope with this shit. Somebody please lend some advice.

I have the exact same problem after pressure cooking when the vials are sealed with tin foil and then go into a toaster oven to dry them out. Does drying them out somehow create the floaters out of plain tap water?
 
Just rinse in alcohol no tap water tap water has calcium in it which could be the white floaties you see heat will evap the alcohol

Sent from my SAMSUNG-SM-G891A using Tapatalk
 
Bro dont fuck with the vials. You can buy sterile vials that are sealed. Thats the one item i dont bother cleaning.

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Alcohol can leave a residue too

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I had this problem too but mine whats the geat eating the media reciever. I didnt realize how important glass reciever was.
 
Why don't u clean with alcohol and let it evaporate till dry? I clean my body of syringe all the time with alcohol and put new needle. 91% alcohol that is. I draw my gear and squirt some back in vail to get air out and I never have floaters.
What I am saying is tap water is not sterile so why are u using that?
Alcohol 91% that are hydrogen peroxide is what u should soak your stopper in, so why not for veils?
If u gonna heat in oven that should kill stuff too.
I would heat in oven let cool in clean area then rinse with alcohol and let dry in clean area.
If u using glass media bottle to filter into why don't u use same procedure u using the sterilize it.

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Heating any liquid can create I residue. Especially that u doing it face down. Where u want it to evaporate?
 
Don't use tap water create a top foil cover to keep anything from falling inside vail. Or use anything to make top. Don't put face down leave room to any thing cooked if u don't and no matter what u do u are cooking it to vail especially with tap water.
Use alcohol.
 
Can't edit post.
Create a top over pan to keep anything from falling in. Top would leave room on sides to allow vent heated things always rise. With vails upside down u trapping the rise process cooking it onto vails.
If u want overkill use boiling distilled water and then rinse with alcohol that should do it.
If u wanna bake imo u gotta leave room for any residue to escape. My 2cents but I never did this. I do fill my body of syringe up 3 times with alcohol and squirt out and wait to dry. I have done this for years with new needles and never had an infection or anything get back into vail leave floaters.
 
This is a super overkill method I would not go to this extent but here we go.
1-boil distilled water sterilize vials.
2-rinse vials with alcohol
3-have your pan set up with a top section with open sides to vent anything that needs to rise with heat
Put vials into oven. 338f-60min 320f-120min. 302f-150min 284f-180min 250f-12hours.
Be sure your bottles are made of borosilicate shock proof to temp changes. Let cool in area with top over pan and a vent on venting air out. Not closes on sides though u gotta let temperature changes allowed to happen without trapping condensation.
4-have your stoppers soaked in 70%, then allow to dry.
Put rubber stopper on vial to keep sterile.
STOP USING TAP WATER POINTLESS TO RINSE WITH SOMETHING NOT STERILE
Use alcohol for last rinse.
 
I have always bought sterile sealed vials. I peel metal piece off and recrimp new one over same stopper. So vials stays sterile and nobody questions u about there being no safety flip top.
 
Muscle mechanic said:
I have always bought sterile sealed vials. I peel metal piece off and recrimp new one over same stopper. So vials stays sterile and nobody questions u about there being no safety flip top.
Click to expand...

That's not a bad idea. I never though of that. Just recrimp over the stopper.


The way I figure is there is always air coming in contact with something at some point. Even if it's just when you're drawing from the media receiver. Best thing is to just minimize it I think.

I still don't think theres anything wrong with the sterile shrinkwrapped vials. Once you're ready to use them cut open the plastic and just take them out one at a time. I even take the rubber stoppers and flip tops out one at a time.

I draw 30ccs at a time out of the media receiver and fill 3 vials and just put the cap back on until i'm ready to draw more. I put them all in a row with the stoper in and top on and crimp them all at the very end.


Once I figured out the right bottle top to use it was smooth sailing. I just happened to get really unlucky and the first two bottle tops I bought were fucked up so it had me questioning everything lol.
 
frizzleman said:
I have been trying to get this shit right for a LONG LONG time now. INCREDIBLY frustrated. This is what I do.


Bathe Vials in Rubbing Alcohol.
Rinse with Tap Water 6 times
Put face down on pan lined with tin foil
Place in Oven at 450 for 2 hours (overkill for sterilization)

No matter how many times I rinse these fucking vials as soon as they come out of the oven and something goes into them there are white floaties. 90% of the Vials look contaminated with shit. I thought maybe my filter was shitty so I took a vial right from the oven and put poland spring water in them and the same damn thing. White floaty bullshit in the vials.

I have no idea what I am doing wrong but its getting completely exhausting. Please dont just get on here and tell me to use pre-sterilized vials... that does not help.

Does tap water somehow leave white floaty bullshit in the vials? Cant see how since I hold new vials after the Alcohol bath up to a light with tap water in them and they are completely clear with no floaters. Somehow after the oven, most vials are trash. I am at the end of my rope with this shit. Somebody please lend some advice.

I have the exact same problem after pressure cooking when the vials are sealed with tin foil and then go into a toaster oven to dry them out. Does drying them out somehow create the floaters out of plain tap water?
Click to expand...
1- They make special detergent for labware 2) USE DISTILLED WATER ONLY 3) CLEAN & SOAK IN METYL ALCOHOL (YELLOW HEET) 4) LET VIALS DRY UPSIDE DOWN.
HONESTLY- BUY PREMADE. I don't feel your knowledge base of basic aseptic technique(s) is strong enough. I learned by trial and error but never had 'floaties' unless an experiment craashed. I'm looking out for your well being not trying to put you down.
 
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