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    Default Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats


    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats, PJ Apel, Muscle & Nerve Volume 41, Issue 3, pages 335–341, March 2010

    Given the wide-ranging effects of IGF-1 on nerve regeneration and the known decline in IGF- 1 with age, we sought to determine whether IGF-1 replacement would improve nerve regeneration in aged animals. Previously, we found that increasing systemic IGF-1 via pulsatile growth hormone injection did not improve nerve regeneration (unpublished data).Thus, the purpose of this study was to examine the effects of IGF-1 locally delivered to a nerve transection site in aged rats, and to evaluate neuromuscular recovery, axonal regeneration, myelination, Schwann cell activity, and the resulting changes at the NMJ.

    Here's how they delivered it... it wasn't cell made IGF-1 but administered IGF-1 near site continuously.



    Discussion

    In this study we sought to determine whether IGF-1 would improve neuromuscular recovery after nerve injury during aging. We found that IGF-1 improved nerve regeneration by acting on the axons and Schwann cells, and secondarily on the NMJs. These results suggest that there is no loss of sensitivity to IGF-1 with age and that IGF-1 can improve regeneration after nerve injury during aging.

    Previous studies have examined the effect of systemically administered IGF-1 during neuromuscular recovery in neonatal rats. Vergani et al. found that systemic IGF-1 promoted neuronal survival following crush injury and improved muscle reinnervation.34 Kanje et al. found that locally delivered IGF-1 following crush injury in juveniles led to improved sensory function.35 Tiancgo et al. found that IGF-1 locally delivered to an end-to-side repair improved muscle function in young rats.36

    To the authors’ knowledge, the current study is the first to examine the effect of IGF-1 during nerve regeneration in aged animals. Axonal outgrowth is central to nerve regeneration, and IGF-1 is a well-documented promoter of motor neuron survival,37 axonal growth,38 and axonal branching.18 In the regenerating nerve, IGF-1 is a key promoter of initial sprouting and subsequent elongation of axons39 (for review, see Rabinovsky2). Although there has been extensive investigation of the effect of IGF-1 on neuron outgrowth and survival, nearly all of the studies were performed in vitro or in embryonic animals. Thus, it is not known how increased age affects the neuronal response to IGF-1. In this study we found that, for metrics of neuronal trophism, aged animals had a robust response to IGF-1 treatment, increasing axonal number, diameter, and density in the regenerated nerve segment. Thus, the neuronal response to IGF-1 of the regenerating axon does not appear to be limited due to age. The Schwann cell is a key factor in promotion of nerve regeneration. The effect of IGF-1 on Schwann cells has also been studied. IGF-1 is known to promote Schwann cell survival,40 motility,41 proliferation, 42 and myelination.43 However, the physiology of Schwann cells from aged animals is largely unknown. This study has addressed some of the parameters of Schwann cell function in vivo, particularly myelination and GAP43 expression. GAP43 is upregulated in nearly all myelinating Schwann cells of the distal stump following axotomy.31 Expression of GAP43 is postulated to play a role in the interaction between the regenerating axon and Schwann cell.30 In this study, we found that GAP43 expression was significantly upregulated in both young and aged animals with IGF-1 treatment. In addition, GAP43 expression was higher in aged animals treated with IGF-1 than in similarly treated young animals, suggesting that age increases sensitivity to IGF-1 for this aspect of Schwann cell function. However, due to pooling of the whole muscle mRNA, in this study we could differentiate between an increase in Schwann cell number and an increase in activity. Furthermore, because the sample was from muscle, the increase in GAP43 expression was due to intramuscular neurons, terminal Schwann cells, or both. GAP43 expression in the regenerated segment itself was not assayed. In addition to GAP43 activity, IGF-1 increased myelination in the regenerated nerve, independent of the increase in axon diameter. Thus, at the Schwann cell level, age does not impair the response to IGF-1 and results in an increased regenerative response.

    Failure to achieve reinnervation of the muscle is thought to contribute to impaired neuromuscular recovery after nerve injury.44 In this study we found by histologic examination that the motor endplate regained an innervated, pre-injury morphology in all but the aged saline group. This failure to reinnervate may have been due to delayed nerve regeneration or instability of the postsynaptic NMJ, both of which have been shown to occur with aging.9 In this study, treatment with IGF-1 may have accelerated axonal regeneration, leading to earlier reinnervation of the muscle, and thus preservational of the postsynaptic NMJ. One limitation of our study is the lack of functional and electrical assessments. Functional recovery is the gold standard for clinical outcomes in human subjects, but, historically, functional recovery in rodent models has been difficult to assess.45 In rats, there is a poor correlation between functional, electrophysiologic, and histologic outcomes. 46 In this study, electrical measures of function were performed and showed a trend toward improvement with IGF-1 treatment, but this was not statistically significant (data not shown). Nonetheless, we were able to demonstrate that IGF-1 improved nerve regeneration in aged animals. An important strength of our study is the in vivo comparison between young adult and aged rats after a complete nerve transection. Previous studies of IGF-1 on nerve regeneration utilized in vitro, neonatal, or juvenile animal models. In addition, many previous studies utilized crush injury models. Crush injuries in rodents typically have a near 100% rate of recovery and do not accurately model clinical scenarios wherein neuromuscular recovery is often very poor. Although previous studies have helped to answer many questions about the effects of IGF-1, this study is the first to examine in vivo the effects of age and IGF-1 on neuromuscular recovery following nerve transection.

    Overall, our study has provided substantial evidence that locally delivered IGF-1 improves neuromuscular recovery after nerve injury in aged animals. We showed that, when local IGF-1 levels were equally supplemented in young and aged animals, nerve regeneration and NMJ preservation were similar, suggesting that IGF-1 may be a contributing factor in age-related impairment of neuromuscular recovery. However, our study has not conclusively demonstrated that IGF-1 deficiency is the immediate cause of age-related impairments in neuromuscular recovery after injury.
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    I couldve told you this stuff is a miracle drug with nerve problems, but it makes me very happy to see the medical community catching on to its tremendous benefits
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    Quote Originally Posted by The Dude View Post
    I couldve told you this stuff is a miracle drug with nerve problems, but it makes me very happy to see the medical community catching on to its tremendous benefits
    Gives validation to those that want the science!

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    yep, thats how i came to find igf-1 lr3, for nerve damage, and ive told the story here countless times , so i wont get into it, but its out there, and yes it was a miracle cure for my nerve regeneration many many years ago
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    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    can someone tell me how to read mcg on a insulin needle I cant figure it out and I don't want to mess up my dosage? and how long can I stay on igf-1 lr3
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    lol ok so on the needle where its says .5 that would be 50 mcg? and where its says 10 it would be 100 mcg? god this stuff is hard as hell or im just a big retart?? lol
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    Quote Originally Posted by pitbull1125 View Post
    lol ok so on the needle where its says .5 that would be 50 mcg? and where its says 10 it would be 100 mcg? god this stuff is hard as hell or im just a big retart?? lol
    Can you post a pic?

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    Quote Originally Posted by pitbull1125 View Post
    lol ok so on the needle where its says .5 that would be 50 mcg? and where its says 10 it would be 100 mcg? god this stuff is hard as hell or im just a big retart?? lol
    NOOOO!! Don't do that. 1 full syringe is 1000mcg. So .1 is 100mcg. So the 5th tiny line is 50mcg.
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    ok great so each tiny line is counted by 10 right?
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    Quote Originally Posted by pitbull1125 View Post
    ok great so each tiny line is counted by 10 right?
    That's how it is with the insulin syringes I use for igf -1. Each increment is 10 mcg; the "5" equals 50 mcg. It's roughly 1/4" in the barrel.
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    yeah multiply everything by 10 to get mcg dosage, though someone the other day posted a pic of a syringe that was half the size only .5ml so with that syringe you would multiply by 20, but ive never even seen those syringes until it was posted here
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    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    great study on nerve regeneration with insulin-like growth factor-1
    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    Quote Originally Posted by EMW14 View Post
    That's how it is with the insulin syringes I use for igf -1. Each increment is 10 mcg; the "5" equals 50 mcg. It's roughly 1/4" in the barrel.

    https://www.musclechemistry.com/uploa...confusion.html

    That Link Above also has Syringe photos with measurements to help show micrograms per syringe tick mark for both 100 and 50 I.U. Sterile Syringes
    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    great article
    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    Great information.
    Get It Done!

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    bump
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    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    I had a spinal fusion in June 2015, my c5 & c6 were smashed, the surgeon said he saw some pinched nerves, and right after surgery my arm felt different....I still have about a one inch atrophied left tricep, its really a bummer and has effected my strenght esp on chest and tricep considerably. Wonder if this would help?

    If so, where would I put the IGF, the atrophied tricep, or the point where is was pinched, my back. I'm not crazy about pinning my spine LOL
     

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    Presser or muscle mechanic this should be a sticky'd..........I have been trying to find this info using the search button with no luck ( I know operator error). I have so many friends that have "dead" muscles and I try to explain how IGF works, but this article does it so much better than my limited on the spot knowledge.
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    bump
    Beach bodies are made in the winter.

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    Quote Originally Posted by yellow snow View Post
    Presser or muscle mechanic this should be a sticky'd..........I have been trying to find this info using the search button with no luck ( I know operator error). I have so many friends that have "dead" muscles and I try to explain how IGF works, but this article does it so much better than my limited on the spot knowledge.
    Then We shall STICKY POST IT brutha!

    Appreciate your feedback and suggestion , so yeah lets add it to the sticky threads at the top of this peptide forum!
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    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    Superb reading!
     

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    bumping for new members question on this
    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    so how often and at what dosage would be a good starting point and would a progressive increase be warranted? how long would one take igf-1, even after benefits were noticeable? i haven't tried it yet, because cost as well as length of use being a factor. te reviews i've seen about it have been great.

    i'm talking in a therapeutic manner of use here and not for workout regimen.
    on that note though, what would be good doses for workouts as well.
     

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    30mcg daily as long as your able
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    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    I have some nerve pain. Cant wait for the day I get to try this stuff.
     

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    alright, i ordered it and now going to give it a go.
    thanks presser for the easy transaction.
    fantastic customer service.
     

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    Anyone experiencing nerve muscle damage , this is a solid read, and real healing option !! That's how I was first I introduced to igf-1 and I've never stopped using since!!
    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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    Quote Originally Posted by The Dude View Post
    I couldve told you this stuff is a miracle drug with nerve problems, but it makes me very happy to see the medical community catching on to its tremendous benefits
    yes it is a miracle !
    Effect of locally delivered IGF‐1 on nerve regeneration during aging: An experimental study in rats

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