How to choose right syringe filters and filter membranes

Presser

Presser

Admin
Staff member
Administrator
[h=2]How to choose right syringe filters and filter membranes[/h]
Hi guys,


The texture of syringe filters include Nylon, PVDF, PTFE, MCE, CA, Glass Fiber, PP, PES.


When choosing the texture of syringe filters, we need to consider pore diameter, chemical resistance( if can resist all carrier oils and solvents), oil-based or water-based solution, and etc.


Here are the pore diameter of common syringe filters.


Pore diameter:
Nylon, PVDF, PTFE, MCE, CA, PP, PES: 0.22um, 0.45um
Glass Fiber: 0.7um, 1.2um


Here are some common questions we enounter when choosing proper syringe filters?
1. Is it necessary to have a 0.22 um to have a sterile solution or the 0.45 um gives acceptable results concerning the dangers of bacteria ?
2. What is the average filtering time per 100ml oil-based injections and water-based injections? What carrier oil you use?
3. Warming your injection will make filtering much easier. What is the filtering time difference between warmed and unwarmed oil?
4. Which membrane are the best for a steroid based solution? Oil-based and water-based.


Here is the chemical resistance chart of Whatman syringe filters/filter membranes.


Nylon PVDF PTFE MCE CA Glass Fiber PP PES
Benzyl Alcohol LR R R R LR R R NR




R=Resistant
LR=Limited Resistance
NR=Not Resistant




I would like to answer the above questions with my shallow expertise.
1. 0.22 um is used for sterilizing-grade filtration. Literally speaking, getting rid of bacterials. 0.45um is used for clarification filtration and pre-filtration. Always stick to
0.22 um though more time-consuming. I want to correct my words in the past posts.
4. Just considering the pore diameter, Glass Fiber is out of the game. Then comes to chemical resistance, Nylone, CA, and PES are out of the game too. So the rest
options might be good for use: PVDF, PTFE, MCE, PP. But deeper consideration is: would these syringe filters not be eaten by other solvents(like BB, EO, guaiacol,
PS80), even carrier oils? As far as I know, sesame oil eats PES.


My provisional conclusion is: 0.22 um of PVDF, PTFE, MCE, PP are good for use. But it is a open proposition.


All MC bros are welcome to anwser these questions. Always glad to discuss this with all you guys.




PS:
Nylon: Nylon
PVDF: Polyvinylidene Fluoride
PTFE: Polyfluortetraethylene
MCE: Mixed Cellulose Ester
CA: Acetate Cellulose
Glass Fiber: Glass Fiber
PP: Polypropylene
PES: Polyether Sulfone​
 
Here is the link of Whatman chemical compatibility chart. Please check this out:http://www.gelifesciences.com/gehcls...1015100012.pdfLR means that Nylon and CA has Limited Resistance to BA. My understanding is that BA will melt Nylon and CA after you filter a certain amount of injections. But nobody knows where the breaking point is. To avoid uncertainty, we can use other texture for replacement. I know that my conclusion might be different to what you saw online in the past. But our thoughts need to be updated all the time. I was wrong about this in the past too. Nearly all threads said that Nylon is preferred for filtration. NR means that PES is Not Resistant to BA. BA wil melt PES for sure.@Muscle mechanicYes. 0.22 um of PVDF, PTFE, MCE, PP are good for us.For bottle tops, I would recommend PVDF, PTFE, MCE, PP too. Glass units is also good for use. Here is how you can sterilize glass media bottles: 1. Wash with 70% medical alcohol. 2. Sterilize it with an autoclave.
 
Maine Manufacturing makes the zapcaps now and I still love 'em. They are tougher than the Millipore steritops or cups [I had at least 5 leak on me and I've been doing this almost 15 yrs].
Nylon is your best all purpose. If you have 100% EO or want to filter someting like Guiacol so it's 'sterile' you'll want the 'PTFE' membrane (It's similar/partially teflon).
One gripe I get is that other than the cellulose acetate (just say no) zaps are NOT sterile. Easy fix. Have a bottle of 0.22 filtered BA and spray it on the filter surface. Attach vacuum source. Drip,drip,drip... NOW it's sterile ! Use a 0.45 micron first. NEVER, EVER be a tightass and start with a 0.22. You'll eat up filters and waste money. Ideally filtering thru a 0.8 micron and 0.45 micron nylon filter sheets (vacuum flask setup) is best but it takes looking.
Here's a trick. Have some textured gauze. When the flow slows down I suck the liquid out into glassware. I then clean the surface well and microwave the liquid to a reasonable (consult your reference temp for membrane). You will get about 750ml out of 500 -or should unless you are getting powders far downstream). Same goes for the 0.22 Nylon zapcap- I recommend a basic MCT 60/40 (c8/c10) mix. Nobody is getting 500 without pre-filtering. MAYBE with Digycol (840?) which has a viscosity of 10. Grapeseed oil is 54, CSO is 76.
 
The texture of syringe filters include Nylon, PVDF, PTFE, MCE, CA, Glass Fiber, PP, PES.


When choosing the texture of syringe filters, we need to consider pore diameter, chemical resistance( if can resist all carrier oils and solvents), oil-based or water-based solution, and etc.


Here are the pore diameter of common syringe filters.


Pore diameter:
Nylon, PVDF, PTFE, MCE, CA, PP, PES: 0.22um, 0.45um
Glass Fiber: 0.7um, 1.2um


Here are some common questions we enounter when choosing proper syringe filters?
1. Is it necessary to have a 0.22 um to have a sterile solution or the 0.45 um gives acceptable results concerning the dangers of bacteria ?
2. What is the average filtering time per 100ml oil-based injections and water-based injections? What carrier oil you use?
3. Warming your injection will make filtering much easier. What is the filtering time difference between warmed and unwarmed oil?
4. Which membrane are the best for a steroid based solution? Oil-based and water-based.


Here is the chemical resistance chart of Whatman syringe filters/filter membranes.


Nylon PVDF PTFE MCE CA Glass Fiber PP PES
Benzyl Alcohol LR R R R LR R R NR




R=Resistant
LR=Limited Resistance
NR=Not Resistant




I would like to answer the above questions with my shallow expertise.
1. 0.22 um is used for sterilizing-grade filtration. Literally speaking, getting rid of bacterials. 0.45um is used for clarification filtration and pre-filtration. Always stick to
0.22 um though more time-consuming. I want to correct my words in the past posts.
4. Just considering the pore diameter, Glass Fiber is out of the game. Then comes to chemical resistance, Nylone, CA, and PES are out of the game too. So the rest
options might be good for use: PVDF, PTFE, MCE, PP. But deeper consideration is: would these syringe filters not be eaten by other solvents(like BB, EO, guaiacol,
PS80), even carrier oils? As far as I know, sesame oil eats PES.


My provisional conclusion is: 0.22 um of PVDF, PTFE, MCE, PP are good for use. But it is a open proposition.


All MC bros are welcome to anwser these questions. Always glad to discuss this with all you guys.




PS:
Nylon: Nylon
PVDF: Polyvinylidene Fluoride
PTFE: Polyfluortetraethylene
MCE: Mixed Cellulose Ester
CA: Acetate Cellulose
Glass Fiber: Glass Fiber
PP: Polypropylene
PES: Polyether Sulfone



Believe it or not the Foxx 0.45 PES can take 250mg (1% BA, 10% BB) enanthate in GSO or MCT oil like a it's a big gulp. I wouldn't rely on 0.22 but you'd probably be OK with it. I am not sure why nylon isn't rated higher. For chemikaze's using Guiacol or other potent solvents I used to say PVDF but Millipores bottle unit's IMO have been shitty whereas I have beaten the living dogshit out of Zapcaps and they overperform by 50%+ .
PTFE filters strong acids and & aldehydes.
 
zapcap cr coming in 0.45um. Is it enough for proper filtration?



.45 is considered sterile.
And I am pretty sure most labs use It!
Anyway they say certain virus's can get thru but u don't get I viral abscess only bacteria ones!
Ba will stop production of viruses and they will not be preserved in your vial to become active. They need a host or they die!
But still baking is done with. 45 and the time saved u can bake a cake and lots of stuff while oven us hot!

Sent from my SAMSUNG-SM-G890A using Tapatalk
 
hmm, I literally just bought a few more nylon filters because I seemed to have read all over the web that was preferred.

I didn't quite understand the way you worded it. You said nylon and pes are out because of chemical resistance. What does that mean? That solvents will eat the filter?



These links are compatibilty charts. There are some things missing but you will get an idea. PES is only ok for a prefilter of a very low BA/BB mix. Nylon is the best overall but prefiltering ie a 0.8 or 1.0 micron nylon syringe filter or 47mm membrane (all found in nylon) are ideal if you want to save your filters. I am going to put a post dispel a lot of myths are things people are doing wrong.
attachment.php
attachment.php

http://sevierlab.vet.cornell.edu/res...art-Detail.pdf
http://www.treborintl.com/content/ch...sistance-chart

Pics (right) Millipore 0.22 micron PVDF STERIVEX. Prefiltered and using the veterinary syringe NOBODY SHOULD OR NEED TO USE A CAULK GUN. That syringe is $8 at any tractor supply or online. Guys, You've gotta prefilter. It is easier and saves you money. You can't be a cheapskate.
 
Using a 90mm (or whatever diameter the membrane is) nylon or PVDF filter membrane ON top of say the 0.22 micron PES will do the dirty work and should fix this problem. Unless already pre-filtered a 0.65-0.45 micron size will do it. Just look at the drip. If it rolls through like the Wermacht thru Poland you are kind of screwed. PES ones that sell for $13 are not a good buy. Maybe if they are $5 each in a box of 12 (fire sale price) they can be useful. If someone is making a bunch of bacteriostatic water or filtering GSO, CSO, or MCT oil. It' s not a problem. Just my $0.02.
 
Quick question

Can someone explain the differences between inlet outlet female male?

Getting some filters and they have female inlet and male outlet. Are these OK to use? I have PDVF 0.22um 33mm and PES 0.10um 33mm both sterile. The first used for oils and the second for amino acids in water.

Thanks



I think that is the normal way. The syringe goes in, or screws to the 'female' top and the male has the 'dong' for lack of a better term at the end. PES should be fine for aminos. It just depends on what the preservative/anti-microbial is (parabens, ect) .
 
zapcap cr coming in 0.45um. Is it enough for proper filtration?



Cellulose Acetate is not anywhere near ideal. If you had a 70mm membrane set on top of it that would help a lot. That's the size they come in. Hard to come across unless you look quite a bit. And never use Nalgene Nylon filters. They suck and Foxx bioscience is weak too (membrane is not very thick). At least with those they take 90mm membranes. Watch the flow rate. It should be a steady drip (quick) not like sucking Pepsi through a straw.
 
Keep in mind this is referencing SRTAIGHT BA, BB, ect. so even the wimpy PES can handle some things in small amounts but I would avoid altogether. Nylon: It gets a bad rap. It's a good membrane as long as them membrane is adequately thick
.
PDFE - there are hydroPHILIC and hydroPHOBIC versions- that being said this is for filtering PURE SOLVENTS. You can use it but I can tell you this much, use it in syringe filters and get out a fucking sundial.

PVDF: All purpose, hydrophilic, and is good. Nylon is cheaper , and for the most part is perfectly ok. For a syringe use PVDF gets the nod.

MCE: I'm not sold on it. Have no feedback.
AS ALWAYS DO RESEARCH AND PREFILTER. DON'T SPEND $6.00 TO FILTER 30CC OR RUIN YOUR THUMBS. I have no idea why I see dudes wasting $$ using 0.22 only. YES-use them, but find a larger pore and you will have a much easier time. Spend a little to save a lot of money and effort. Hint -ebay.
 
You must log in or register to reply here.

Similar threads

big_iron_up
How to choose right syringe filters and filter membranes
2
Replies
39
Views
24K
Presser
Presser
Back
Top