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    Default How to sterilize vials caps

    Another question lads - how to sterilize rubber vial caps? IPA bath + oven 250F 30mins, is it sounds good? What is melting point of rubber caps?
     

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    Eh I would wash with anti bacterial dish soap them rinse really well. Then I put them in, sorry I can't think of the name of the machine right now all's it is, is a jewelry cleaner that sends little impulse waves to get jewelry clean but I fill it with 91% isopropyl alcohol and run it a couple times then I pour out the alcohol and let them dry in the rack that it comes with. If someone knows what I'm talking about please post the name. I think its just an ultrasonic cleaner. The 91% IPA will kill off everything after being washed in antibacterial soap and I also give them a rinse in ba to prevent the growth of new bacteria when I'm done and never in 15 years or so have ever had an issue and you really don't need to rinse in ba either but use non latex rubber gloves to handle them. I pour IPA on the gloves, just a little before I touch them. You want to hear you bottles after basically doing the same process of washing rinsing all soap the soak in 91% IPA, then I use a toaster oven to heat bottles at 350F for 35 minutes then let them cool inside the oven then I immediately put a rubber stopper and cap on them as soon as I pull them out. No matter what you do air is going to touch it but its fine. Then when my solution is ready I use my pippettes to fill from media bottle and transfer the the smaller bottled I just use new gloves on a clean surface with paper towel down and flip the cap and stopper off without touching the stopper if I can but the gloves I have are clean and sterile each time and fill them the re cap and crimp.

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    thanks for info. I was thinking about soap + IPA but wasnt sure thats will be enough. Will add on BA to the process as you mentioned.

    For vials I found this:
    ~Wash vials in HOT soapy water
    ~Rinse in HOT water
    ~Rinse in distilled water
    ~Rinse twice in alcohol
    ~Bake vials upside down at 350 for 30 minutes.
    ~Vials remain upside down until gear is ready to be filtered into them.
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    Yeah, it's pretty good to rinse in alcohol
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    Bro I am giving them bath in dish soap than wrap them in kitchen foil put to pressure cooker for 25min ,after that I bake them for dry in oven at 150c . When vials are filled I am opening the foil and fit them on top of vials
    Never have any problems with unsterile gear
     

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    I have try to bake rubber stoppers in oven at 120C but could feel wierd smell like burnt rubber once open the foil
     

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