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    Default Looking for help with electric vacuum and bottle top filtration process

    Ive been using syringe filters and whilst making 100ml isn't too bad, its still a bit of a ball ache. A couple of hours from start to finish for 150ml the other night wasn't much fun really when I keep hearing about bottle top filtration.

    Im planning on getting some zapcaps, an electric vacuum pump and some glass media bottles. I also understand that pre filters are a good idea?

    The parts that I don't understand is firstly if the pump that Im looking at is the correct type for the job: https://www.ebay.co.uk/itm/Vacuum-Pum...-/112403227095

    Secondly, I don't know what tube, what size the tube should be and where the tube connects to on the machine and the set up?

    Thirdly, Im thinking that once the oil has been filtered and is now in the media bottle/receiver flask, how do you keep it sterile? When I take the filter off from the top of it, it will then be exposed to air which will then make it unsterile? I think I read that you can buy a special rubber top for the media bottle so you can then get the oil out of the bottle and into whatever vials you want to store it in. Can anyone explain how that part is done?
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    I have the same pump like you. With that pump i use a glass bottle top. They are 3 parts (the flask receiver, the top part and a middle part where you will put your filter). For the filter i use pvfd filter disc of 50mm. I am
    Able to filter around 150ml in a time without the filter getting clog and after that the filter start to get clog and i advise to replace it. Also my filters are from china and that can be the reason for them to get clog after 150ml, but they are 30$ per 50 filters, not really expensive. The speed i get is around those 150ml in about 15-20 min. One time is filtered i leterally disassembly the bottle top filter and i distribute the oil in vials of 10ml with a glass pipette. All the material gets sterilize for every use. You can filter first for example testo e, and then distribute in vials and then you can filter the next hormone like for example deca. Nothing is gonna happen, no worries. Same process and then the next hormone and over and over again. Another tip that i can tell you is to warm your solution to 220-230F for about 10 min. Then you will be sure that all bacteria will be dead and if for some reason they pass thru the filter, no worries, nothing is gonna happen, as those possible bacterias that could pass thru the filter are dead and a dead bacteria is not gonna create infection. Also you solution will have around 2% BA so no bacteria will also grow there.

    In the links you can take a look to my glass bottle top system and filter:

    https://s.aliexpress.com/6jMzQBZB
    https://s.aliexpress.com/eEVJbUrA

    If you are gonna use whatman zapcap, i recommend you a peristaltic pump and a flask receiver

    Use 0.22um filters are slower but it doesn't exist bacteria so small, instead if you use a 0.45um filter could be the case that some bacteria will pass thru the filter.

    Good luck and i hope i help you enough


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    Quote Originally Posted by Giru View Post
    I have the same pump like you. With that pump i use a glass bottle top. They are 3 parts (the flask receiver, the top part and a middle part where you will put your filter). For the filter i use pvfd filter disc of 50mm. I am
    Able to filter around 150ml in a time without the filter getting clog and after that the filter start to get clog and i advise to replace it. Also my filters are from china and that can be the reason for them to get clog after 150ml, but they are 30$ per 50 filters, not really expensive. The speed i get is around those 150ml in about 15-20 min. One time is filtered i leterally disassembly the bottle top filter and i distribute the oil in vials of 10ml with a glass pipette. All the material gets sterilize for every use. You can filter first for example testo e, and then distribute in vials and then you can filter the next hormone like for example deca. Nothing is gonna happen, no worries. Same process and then the next hormone and over and over again. Another tip that i can tell you is to warm your solution to 220-230F for about 10 min. Then you will be sure that all bacteria will be dead and if for some reason they pass thru the filter, no worries, nothing is gonna happen, as those possible bacterias that could pass thru the filter are dead and a dead bacteria is not gonna create infection. Also you solution will have around 2% BA so no bacteria will also grow there.

    In the links you can take a look to my glass bottle top system and filter:

    https://s.aliexpress.com/6jMzQBZB
    https://s.aliexpress.com/eEVJbUrA

    If you are gonna use whatman zapcap, i recommend you a peristaltic pump and a flask receiver

    Use 0.22um filters are slower but it doesn't exist bacteria so small, instead if you use a 0.45um filter could be the case that some bacteria will pass thru the filter.

    Good luck and i hope i help you enough


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    Have two questions for your brother as I am currently using Nalgene rapid flows with a glass media bottle I can take the PSI up to about 13 or 14 and filter 500ml in about 30 minutes however each bottle top filter across between 13 and $15. My questions are the filter paper you buying for your glass setup sterilized filter papers? My second question is the pipette you were using to fill your 10ml bottles do you have a link or a picture you could show me? I have my eye on one I will post a link for it in a minute and you can tell me what you think. Someone told me to buy the one I'm going to post a link to now I just can't figure out which stirile plastic pipettes to put inside of it.

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    Quote Originally Posted by bigmills View Post
    Have two questions for your brother as I am currently using Nalgene rapid flows with a glass media bottle I can take the PSI up to about 13 or 14 and filter 500ml in about 30 minutes however each bottle top filter across between 13 and $15. My questions are the filter paper you buying for your glass setup sterilized filter papers? My second question is the pipette you were using to fill your 10ml bottles do you have a link or a picture you could show me? I have my eye on one I will post a link for it in a minute and you can tell me what you think. Someone told me to buy the one I'm going to post a link to now I just can't figure out which stirile plastic pipettes to put inside of it.

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    Hi bigmills,

    the filter discs papers i really don't know if they are sterilized or not, but i am using them for more than 2 years and i never ever had a problem, neither other people that used my gear. As i don't know if they are sterilized or not and i don't have an autoclave(i sterilize system and vials with the oven) that is why i keep warm my solution for 10 min at 230F to be sure no bacteria will survive.

    This is the pippete is like this:
    https://m.aliexpress.com/amp/item/32804338389.html


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    Quote Originally Posted by Giru View Post
    Hi bigmills,

    the filter discs papers i really don't know if they are sterilized or not, but i am using them for more than 2 years and i never ever had a problem, neither other people that used my gear. As i don't know if they are sterilized or not and i don't have an autoclave(i sterilize system and vials with the oven) that is why i keep warm my solution for 10 min at 230F to be sure no bacteria will survive.

    This is the pippete is like this:
    https://m.aliexpress.com/amp/item/32804338389.html


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    Hey brother do you know what kind of filter membrane you are using? I'm wondering it it is cellulose acetate and not nylon?

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    Giru is that filter unit from Ali keep tight??
     

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    Yeah, it keeps tight enough. i really like this system,in fact i have 2 of them, one of 250ml and another of 500ml, but i am going to change to peristaltic pump and whatman cap, as i need go faster
     

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    Will give a try then. I found sterile pvdf 47mm membranes, more expensive but all together still much cheaper than ready filter units.
     

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    I also use the membrane PVDF filters that yo can find in aliexpress. They are like 30$ i think for 50 filters. If you have questions you can always pm
    Me


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    Quote Originally Posted by Giru View Post
    Yeah, it keeps tight enough. i really like this system,in fact i have 2 of them, one of 250ml and another of 500ml, but i am going to change to peristaltic pump and whatman cap, as i need go faster
    Hey brother I know you were looking for Speed, but I'm pretty sure you do not use disposable bottle top filters. I would suggest giving one a try hooked to a 1000 ml hybex media bottle. If you go with the Nalgene rapid flow bottle top filter you can filter 1000 ml in way less than 30 minutes and that is using a hand pump, and you do not have to sit there and keep on popping it like you would think. If you do use a disposable like the one I just mentioned I would stay away from the whatman zap cap only because I heard a lot of good things about them however when a friend purchased them they didn't have any threats to screw onto the media bottle.
    When I first started looking into these five or six years ago I thought they were basically all the same maybe a little difference in each one but nothing serious. Well I was definitely wrong I don't know if you read my post the other day about bottle top filters and how the Nalgene rapid flow uses the whole size of the filter membrane to filter and not just the middle. In fact what I would suggest you do if you are interested that is, is go to YouTube and type in Nalgene rapid flow, and you will see what I mean by the quick demonstration about how it uses the whole size of the filter membrane. A few nights ago a friend of mine used a .22um nylon Fox autofill and I would say it probably took him about an hour to filter 750 ml. Then I told him the same thing about the Nalgene rapid flow using a point to 2 with a nylon membrane again and he was able to filter 1000 ml in a little less than 30 minutes. Also that was off of a 500 ml bottle top filter which is only supposed to filter 500 ml but he figured if it's still working why not keep trying and he was able to get 1,000 through it no problem, and if he had a had more oil I think he could have got up to between 12 and 1500 ml through it which is pretty impressive.. don't get me wrong I know they are big and I know it sucks spending $13 on each one. However if you were to hook a payralistic pump to one of those you could probably do 1000ml in 15 to 20 minutes. Another thing he was very worried about blowing the filter alp by taking the PSI too high, so the first 500 mL he was at 15 psi then after that he cranked it up to between 19 and 20 which honestly I have never heard of or seen he actually had to take a video and send it to me for me to believe it.. with a Nalgene rapid flow and an electric pump at 19 to 20 psi you are moving brother. Again I know well I think I know that you don't usually use these disposable wants but I just figured I would throw it out there and see if maybe you would give it a try and get back to us with your experience since you have so much knowledge..

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    Ey Macho,

    Thanks a lot for your post!! I find it very valuable. I have to say that i heard about the nalgene rapid flow and i thought a long time ago to try then. When i started with this i went directly to a bottle top filtration system instead of syringe filters, but i bought directly a glass filtration bottle top due to all the complains i read about people cracking the dispensable ones. In my case, i live in EU and it was not really easy to find the nalgene at good price. I could order from usa, but normally everything that comes here from USA is stop at customs by the shipping companies just to charge you handling and import tax. It is true that the whatman caps don't have nothing to attach them to the flask receiver, basically you have to leave then on the neck's top of the flask receiver(is the only thing i don't like). If i find a interesting price for the nalgene rapid flow here in EU i will give them a try, but i bought 2 days the peristaltic pump(they are not really cheap) hehehe, but i am really interesting to give a try to those nalgene. For the rest of the people that can get then easily i suggest to use them as i believe thay can be really fast and if they work so good with a hand pump, it is really a good deal as an electric pump is also not really cheap.

    Again Macho thanks for your info and your experience. As soon as i get my peristaltic pump i will post a pic for you guys. A good thing that a peristaltic pump also has is that it can also dose the gear in 10,20,30 or whatever ml you program it to do it


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    Hi giru how are you brother .I really need your help can you help me with a recipe for winstrol suspension 100mg/ml and I want to brew like 50ml-100ml at a time .Can you tell me step by step instructions
    Thanks in advance brother.
     

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    Quote Originally Posted by Giru View Post
    Ey Macho,

    Thanks a lot for your post!! I find it very valuable. I have to say that i heard about the nalgene rapid flow and i thought a long time ago to try then. When i started with this i went directly to a bottle top filtration system instead of syringe filters, but i bought directly a glass filtration bottle top due to all the complains i read about people cracking the dispensable ones. In my case, i live in EU and it was not really easy to find the nalgene at good price. I could order from usa, but normally everything that comes here from USA is stop at customs by the shipping companies just to charge you handling and import tax. It is true that the whatman caps don't have nothing to attach them to the flask receiver, basically you have to leave then on the neck's top of the flask receiver(is the only thing i don't like). If i find a interesting price for the nalgene rapid flow here in EU i will give them a try, but i bought 2 days the peristaltic pump(they are not really cheap) hehehe, but i am really interesting to give a try to those nalgene. For the rest of the people that can get then easily i suggest to use them as i believe thay can be really fast and if they work so good with a hand pump, it is really a good deal as an electric pump is also not really cheap.

    Again Macho thanks for your info and your experience. As soon as i get my peristaltic pump i will post a pic for you guys. A good thing that a peristaltic pump also has is that it can also dose the gear in 10,20,30 or whatever ml you program it to do it


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    Sorry brother I forgot you were over in the EU if you don't mind me asking how much is a 500ml nylon Nalgene rapid flow bottle top filter over there?here it is between 13 and $15 however you can easily run anywhere between 1000 and 1500 ml through that filter before it goes bad or start to slow down. With the fox autofill or the Millipore bottle top units I was never able to bring the PSI above say twelve maybe thirteen well the other night using the Nalgene rapid flow I actually took it all the way up to 20 maybe 19 and did not have a problem at all.. I would love to give you as much knowledge as I could brother because you helped me out a lot with a knowledge you have. Also I do have 3 of the wattman zap caps that don't have any threats and I'm kind of confused as to how to get them to stick to the media bottle being as though I do not have a clamp?

    I don't know if you guys have a store over there a hardware store called Harbor Freight but they have really cheap pumps there also did your pump come with a PSI gauge?

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    Quote Originally Posted by deepak View Post
    Hi giru how are you brother .I really need your help can you help me with a recipe for winstrol suspension 100mg/ml and I want to brew like 50ml-100ml at a time .Can you tell me step by step instructions
    Thanks in advance brother.
    I don't do winny suspension at 100mg/ml but i do at 50mg/ml. Be sure that the winny suspension is really painfull man!


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    Quote Originally Posted by Macho313 View Post
    Sorry brother I forgot you were over in the EU if you don't mind me asking how much is a 500ml nylon Nalgene rapid flow bottle top filter over there?here it is between 13 and $15 however you can easily run anywhere between 1000 and 1500 ml through that filter before it goes bad or start to slow down. With the fox autofill or the Millipore bottle top units I was never able to bring the PSI above say twelve maybe thirteen well the other night using the Nalgene rapid flow I actually took it all the way up to 20 maybe 19 and did not have a problem at all.. I would love to give you as much knowledge as I could brother because you helped me out a lot with a knowledge you have. Also I do have 3 of the wattman zap caps that don't have any threats and I'm kind of confused as to how to get them to stick to the media bottle being as though I do not have a clamp?

    I don't know if you guys have a store over there a hardware store called Harbor Freight but they have really cheap pumps there also did your pump come with a PSI gauge?

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    Thanks for your text bro! I answered your pm, take a look. Respect the shop you mentioned, no, i don't know that store here. The whatman zap caps i also don't know how to attached them to the receiver, i've just leave it on the flask and they don't fall. They also filter quite fast, but to be sure you can always hold it by hand, but i normally leave it standing on the flask's neck


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    Quote Originally Posted by Giru View Post
    Thanks for your text bro! I answered your pm, take a look. Respect the shop you mentioned, no, i don't know that store here. The whatman zap caps i also don't know how to attached them to the receiver, i've just leave it on the flask and they don't fall. They also filter quite fast, but to be sure you can always hold it by hand, but i normally leave it standing on the flask's neck


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    Right but if you do that but I'll save you that I mean just sit it on top or Holding On Top it's going to be hard to create any vacuum pressure don't you think?

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    Ok my friend will that recipe will hold winstrol suspension @75mg/ml
    2%ba
    25%bb
    5%ps80

    - - - Updated - - -

    Or you can help me with a recipe for winny suspension @75mg/ml
     

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    Quote Originally Posted by Macho313 View Post
    Right but if you do that but I'll save you that I mean just sit it on top or Holding On Top it's going to be hard to create any vacuum pressure don't you think?

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    Yeah, with a hand pump or electric pump yeah, almost impossible, but with a peristaltic is not problem, the pump will "push and force" the liquid to pass thru the whatman zap cap


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    Quote Originally Posted by deepak View Post
    Ok my friend will that recipe will hold winstrol suspension @75mg/ml
    2%ba
    25%bb
    5%ps80

    - - - Updated - - -

    Or you can help me with a recipe for winny suspension @75mg/ml
    That recipe is good, but in my experience the needle gets clog sometimes and the winstrol don't go thru. I recommend you add also peg300/400, then the winstrol will be easier to go thru the needle.

    Another tip is to use physiological saline instead of distilled water and also i will recommend you to measure the ph of the final product, as that winstrol recipe results in a very acid gear that inside your body will irritate your skin a lot and make it red and very sensitive to pain for around 3-4 days. At least that is my experience

    Good luck and comment results with the winny


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    Thank you brother 5%peg 300/400 would be enough for winny suspension 75mg/ml ???
     

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    Call me crazy, but i use 20% peg400 hahahaha. It works like a charm! My recipe is 25% BB, 2% BA, 2% PS80, 20% peg400, the rest Physiological serum


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    So with your recipe it can hold winny suspension @100 mg/ml ???
     

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    No bro. I don't know that. I do mine at 50mg/ml. I am not a big fan of big concentrations. Normally they are painfull in oil, i dont wanna imagine that in suspension ehehhe


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    No problem bro .I will try for 75mg/ml .I hope it will work with the recipe you gave me.
     

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    Do you mix ps80 in physiological serum separately or you put BA,BB,peg 400, winny raw,ps80 in beaker.i asked bcoz some people mix ps80 in water and some not.
     

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    Deepak i mix winny,ps80,peg400 and until get a clear solution at about 320F. Then let it cool down. When it is at 100-90C ii filter it to a 250ml bottle, add ba and the the serum


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    Quote Originally Posted by Giru View Post
    Deepak i mix winny,ps80,peg400 and until get a clear solution at about 320F. Then let it cool down. When it is at 100-90C ii filter it to a 250ml bottle, add ba and the the serum


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    Close the bottle with the screw and shake like crazy for 5 min. Then is done


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    Guys , how about filter membranes in Nalgene Rapid Flow ? Is it possible to get a PTFE - type ? Im using lot of EO , also guaiacol ,peg 400, and trying my best to prevent filter melting after contact with solvents/cosolvents. How about those Nalgene Membranes , will they stand all solvents/cosolvents without melting a membrane ?
     

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