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    Default SUGGESTIONS FOR CLEANING GLASSWARE PER SIGMA-ALDRICH & COMPANY

    This is a post in response to a thread on the main Anabolic forum where (_____________________) posted and showed pics of some horrendous UGL conconction that some Jabroni UGL used Phisohex (chlorhexidine) to somehow clean glassware. This is most of a PDF from the Sigma-Aldrich company with guidelines on how it is done. This is the URL : https://www.sigmaaldrich.com/content...bull_al228.pdf
    I am leaving out some paragraphs as it gets more academic for my tastes. So, I'm going to shill a little for some likes or whatnot. I go by Pez on Outlawmuscle (since 2005'ish) and EL PEZIDENTE here but lost the password. I like this board and want to contribute what I can and what I have spent time researching and have empirical knowledge/experience with. THIS THING IS 4 PAGES LONG- I'm not wasting bandwith as it can be viewed as a PDF online at the above listed URL. This is a very good primer IMO and will answer a lot of questions and dispel assumptions, or worse, BROSCIENCE.

    INTRODUCTION .Good laboratory technique demands clean glassware, because the most carefully executed piece of work may give an erroneousresult if dirty glassware is used. In all instances, glassware must be physically clean; it must be chemically clean; and in manycases, it must be bacteriologically clean or sterile. All glassware must be absolutely grease-free. The safest criteria of cleanlinessis uniform wetting of the surface by distilled water. This is especially important in glassware used for measuring the volume ofliquids. Grease and other contaminating materials will prevent the glass from becoming uniformly wetted. This in turn will alterthe volume of residue adhering to the walls of the glass container and thus affect the volume of liquid delivered. Furthermore,in pipets and burets, the meniscus will be distorted and the correct adjustments cannot be made. The presence of smallamounts of impurities may also alter the meniscus.
    CLEANING: Wash labware as quickly as possible after use. If a thorough cleaning is not possible immediately, put glassware to soak in water.If labware is not cleaned immediately, it may become impossible to remove the residue.Most new glassware is slightly alkaline in reaction. For precision chemical tests, new glassware should be soaked several hoursin acid water (a 1% solution of hydrochloric or nitric acid) before washing.Brushes with wooden or plastic handles are recommended as they will not scratch or abrade the glass surface.

    GLASSWARE CLEANERS:When washing, soap, detergent, or cleaning powder (with or without an abrasive) may be used. Cleaners for glassware includeAlconox®, Dural®,M&H®, Lux®, Tide® and Fab®. The water should be hot. For glassware that is exceptionally dirty, a cleaningpowder with a mild abrasive action will give more satisfactory results. The abrasive should not scratch the glass. During thewashing, all parts of the glassware should be thoroughly scrubbed with a brush. This means that a full set of brushes must beat hand-brushes to fit large and small test tubes, burets, funnels, graduates and various sizes of flasks and bottles. Motor drivenrevolving brushes are valuable when a large number of tubes or bottles are processed. Do not use cleaning brushes that are soworn that the spine hits the glass. Serious scratches may result. Scratched glass is more prone to break during experiments. Anymark in the uniform surface of glassware is a potential breaking point, especially when the piece is heated. Do not allow acidto come into contact with a piece of glassware before the detergent (or soap) is thoroughly removed. If this happens, a film ofgrease may be formed.

    REMOVING GREASE; Grease is best removed by boiling in a weak solution of sodium carbonate. Acetone or any other fat solvent may be used. Strongalkalis should not be used. Silicone grease is most easily removed by soaking the stopcock plug or barrel for 2 hours in warmdecahydronaphthalene.Drain and rinse with acetone or use fuming sulfuric acid for 30 minutes. Be sure to rinse off all of the cleaning agents.
    RINSING: It is imperative that all soap, detergents and other cleaning fluids be removed from glassware before use. This is especiallyimportant with the detergents, slight traces of which will interfere with serologic and cultural reactions.After cleaning, rinse the glassware with running tap water. When test tubes, graduates, flasks and similar containers are rinsedwith tap water, allow the water to run into and over them for a short time, then partly fill each piece with water, thoroughlyshake and empty at least six times. Pipets and burets are best rinsed by attaching a piece of rubber tubing to the faucet andthen attaching the delivery end of the pipets or burets to a hose, allowing the water to run through them. If the tap water isvery hard, it is best to run it through a deionizer before using.Rinse the glassware in a large bath of distilled water. Rinse with distilled water. To conserve distilled water, use a five gallonbottle as a reservoir. Store it on a shelf near your clean-up area. Attach a siphon to it and use it for replenishing the reservoirwith used distilled water.For sensitive microbiologic assays, meticulous cleaning must be followed by rinsing 12 times in distilledwater.
    STERILIZING CONTAMINATED GLASSWARE: Glassware which is contaminated with blood clots, such as serology tubes, culture media, petri dishes, etc., must be sterilizedbefore cleaning. It can best be processed in the laboratory by placing it in a large bucket or boiler filled with water, to which1-2% soft soap or detergent has been added, and boiled for 30 minutes. The glassware can then be rinsed in tap water,scrubbed with detergent, rinsed again.You may autoclave glassware or sterilize it in large steam ovens or similar apparatus. If viruses or spore-bearing bacteria arepresent, autoclaving is absolutely necessary.

    HANDLING AND STORING: To prevent breakage when rinsing or washing pipets, cylinders or burets, be careful not to let tips hit the sink or the water tap.Dry test tubes, culture tubes, flasks and other labware by hanging them on wooden pegs or placing them in baskets with theirmouths downward and allowing them to dry in the air; or place them in baskets to dry in an oven2. Drying temperatures shouldnot exceed 140°C. Line the drying basket with a clean cloth to keep the vessel mouths clean.

    CLEANING : As is common practice, clean all glassware before use. Any non-abrasive glassware detergent may be used for hand orautomatic dishwasher cleaning. If using a dishwasher or glassware dryer, care should be taken to be sure the dryingtemperature does not exceed 110°C (230°F). Exposure to dry heat should be minimized.Avoid brushes and cleaning pads which could abrade the glass or damage the coating.
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