Whatman filter question

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WeirdAl

Guest

• May 18, 2003

• #1

After looking at the whatman site, I'm wondering if the "sterile" filters that are sold various places are really sterile. The pictures and descriptions I've seen seem to suggest that they are the nylon filters, but those don't seem to be even available "sterile". Anyone know for sure?

 

mailboxkillR

New member

• May 18, 2003

• #2

not sure I just thought they where for filtering particles and the baking was the sterile process. I would imagine that they are as sterile as a pin is by the packaging.

 

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scorpio

Guest

• May 18, 2003

• #3

I believe that they are sealed sterile.

 

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WeirdAl

Guest

• May 19, 2003

• #4

Here is the site I was talking about:
http://www.whatman.co.uk/products/analytical/microfiltration/a_pd_micro_011.html

If you'll notice, none of the nylon filters (and specifically, the 0.45µm NYL) are listed as sterile.

 

gymrat

New member

• May 19, 2003

• #5

I can definetely assure you that they are sterile. My previous job required me to work with cancer cells. Every week I would have to regenerate another set of cells by splitting the week's before in half. Those cells were so sensitive. If everything was not perfectly sterile, they would turn bad on me. The whatman filters are sterile and they are very good at sterilizing as I used them to sterilize my media.

 

gymrat

New member

• May 19, 2003

• #6

WeirdAl said:

Here is the site I was talking about:
http://www.whatman.co.uk/products/analytical/microfiltration/a_pd_micro_011.html

If you'll notice, none of the nylon filters (and specifically, the 0.45µm NYL) are listed as sterile.

Click to expand...

whatman makes many different types of filters. All used for various types of media for cells. You need to get the ones that are sterilized. they work.

 

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WeirdAl

Guest

• May 19, 2003

• #7

Yeah, they definitely have sterile filters, and they certainly look good... I'm just concerned that the nylon filters (which I see for sale all the place, and with various kits) are being sold as sterile, and I can't find any reference anywhere other than those sites selling them that they are offered as sterile. And anyone know what an appropriate filter media would be for filtering hormones, disolved in ba, bb, and oil? The media available as sterile seem to be, with the same pore size (0.45µm):
Cellulose Acetate
Glass Microfibre
Polyethersulfone
Polyvinylidene Fluoride

Also, from looking at this, I notice that some companies are selling the 13mm filters... And what we (I) want are 25mm (since the 13mm seem to have a 10ml volume limit before they are spent).

 

gymrat

New member

• May 19, 2003

• #8

You want the PVDF, 25mm diameter, and .45 micron pore size.

 

gymrat

New member

• May 19, 2003

• #9

you can also use PTFE as long as you are filtering an oil based solution as PTFE are hydrophobic.

 

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WeirdAl

Guest

• May 19, 2003

• #10

Thanks bro!

 

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WeirdAl

Guest

• May 21, 2003

• #11

Thanks again, found that getpinz has the pvdf, not nylon, and at a decent price, too!

 

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WeirdAl

Guest

• May 22, 2003

• #12

And just a follow up, quick delivery, too! Got my order today

 

gymrat

New member

• May 22, 2003

• #13

I just put mine in a minute ago

 

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WeirdAl

Guest

• May 22, 2003

• #14

Cool! I got my order in 2 days!!

 

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NeotonBomb

New member

• May 23, 2003

• #15

To remove 100% of any contaminants including all bacteria use the 0.2 micron filters. That is as sterile as you can physically get your solutions.

 

Last edited: May 23, 2003

gymrat

New member

• May 23, 2003

• #16

It's much more difficult and time consuming to filter through a .2 micron pore size versus a .45 micron pore size. It's not neccessary. The .45 will sterilize your product as that is what I used to divide very sensitive cancer cells at a previous job.

 

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WeirdAl

Guest

• May 23, 2003

• #17

Yeah, I was curious looking at the poor sizes what size would be best, and allow the hormones and oil to pass through just fine. I figure since I'll be sterilizing with ba anyway, this is just an added safety measure, and hopefully will remove most impurities that might be in there.

 

gymrat

New member

• May 23, 2003

• #18

exactly right WeirdAl. The BA will sterilize the solution. the filter is an added extra measure to remove any impurities.

 

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WeirdAl

Guest

• May 23, 2003

• #19

Cool... but I wanted a sterile filter so that I'm not ADDING any impurities with it

 

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joeyc

New member

• May 23, 2003

• #20

I'm getting my hands on 400 of these .3micron filters.
could sell them for @ $2.00 each.

The deal is not hammered out yet.

 

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