Same goes for me. And since we're on the subject--I've been doing some research--you can currently get antibodies to myostatin now, only problem is that they are developed in animals outside the U.S. With the mad cow scare, you now have to have a license to import such materials and do so for research purposes.
For 50 ug it costs about $295 USD. Info about it below (note that this obviously has not been tested much and they say that concentrations should be determined by the end user which means they don't know how much to use):
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Product Name_
GDF8 / Myostatin antibody
Product type_
Primary antibodies
Description_
Cow polyclonal to GDF8 / Myostatin
Immunogen_
His tagged fusion protein (Human).
Cross Reactivity_
Cross-reacts with Human.
Not yet tested in other species.
Tested applications_
Western blot, ELISA
Application notes_
ELISA: Use at a dilution of 1/50000 for antibody at a concentration of 1 mg/ml.
WB: Use at an assay dependant dilution. Predicted molecular weight: 30 kDa in C2C12 skeletal mouse cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Quality is important to Abcam. If GDF8 / Myostatin antibody (ab2121) does not perform as described on this datasheet, a replacement or refund will be offered. Simply notify us within 60 days of delivery using this form.
Research areas_
Cell Biology >> Growth Factors/Hormones >> TGF
Relevance_
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor ß superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.
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Alternative names for GDF8 / Myostatin antibody (ab2121)
Raised in_
Cow
Clonality_
Polyclonal
Purity_
Immunogen affinity purified
Form_
Liquid
Concentration_
1.00 mg/ml
Storage instructions_
Aliquot and store at -20°C or -80°C.
Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GDF8 / Myostatin antibody is on this datasheet. But please do contact us if you would like any reassurance!
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References for GDF8 / Myostatin antibody (ab2121)
General / background references:
Artaza JN et al. Endogenous expression and localization of myostatin and its relation to myosin heavy chain distribution in C2C12 skeletal muscle cells. J Cell Physiol 190:170-9 (2002). PubMed: 11807821
Hill JJ et al. The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum. J Biol Chem 277:40735-41 (2002). PubMed: 12194980
Lee SJ & McPherron AC Regulation of myostatin activity and muscle growth. Proc Natl Acad Sci U S A 98:9306-11 (2001). PubMed: 11459935
If you publish research using ab2121 please let us know so that we can cite the reference on this datasheet.
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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