sarcoplasm
New member
Here is the method:
" it is simply using a 4 molar equivalent of NaOH to the amount of estradiol benzoate present in the solution. I would dissolve about 20g of syno in about 400mL of methanol, filter the binders (through celite using suction), and slowly drop in the 4 molar eq. of NaOH in a 0.5M solution from a buret while stirring the solution. I would let the solution stand (stirring was optional) for about an hour, then pour the solution into about 1L of 1M NaOH solution. After vigorously shaking the solution in a circular motion (to cause the nasty colloid to form very large clumps), I would simply filter it using a coffee filter and rinse a couple times with 1M NaOH, then finally many times with water until the powder and filter were odorless, and the filtrate was colorless. I usually sat the soaking wet filter on a towel to pull as much water out, then stapled it and rubberbanded it to a floor fan to dry for a day or two. I usually recrystallized the stuff from methanol and water after this, but it wasn't necessary as the powder was fairly pure. The whole procedure worked quite well; my genetically gifted, purely mesomorphic friend blew up on the stuff".
My question how much NaOH (in grams) and how much water(in ml's) is in the "4 molar equivalent 0.5M solution? I understand that one must factor ithat the amount of estrodiol being solubilized is 2000 mgs aand also the 0.5M solution is 20 grams NaOH per liter, the part that baffles me is the "4 molar eqivalent" part. Any help would be greatly appreciated
Thanks
" it is simply using a 4 molar equivalent of NaOH to the amount of estradiol benzoate present in the solution. I would dissolve about 20g of syno in about 400mL of methanol, filter the binders (through celite using suction), and slowly drop in the 4 molar eq. of NaOH in a 0.5M solution from a buret while stirring the solution. I would let the solution stand (stirring was optional) for about an hour, then pour the solution into about 1L of 1M NaOH solution. After vigorously shaking the solution in a circular motion (to cause the nasty colloid to form very large clumps), I would simply filter it using a coffee filter and rinse a couple times with 1M NaOH, then finally many times with water until the powder and filter were odorless, and the filtrate was colorless. I usually sat the soaking wet filter on a towel to pull as much water out, then stapled it and rubberbanded it to a floor fan to dry for a day or two. I usually recrystallized the stuff from methanol and water after this, but it wasn't necessary as the powder was fairly pure. The whole procedure worked quite well; my genetically gifted, purely mesomorphic friend blew up on the stuff".
My question how much NaOH (in grams) and how much water(in ml's) is in the "4 molar equivalent 0.5M solution? I understand that one must factor ithat the amount of estrodiol being solubilized is 2000 mgs aand also the 0.5M solution is 20 grams NaOH per liter, the part that baffles me is the "4 molar eqivalent" part. Any help would be greatly appreciated
Thanks
