filling vials consistently

merandom

New member
Ok hello there, SWIM is prety experienced in the field, been reading the forums for quite a bit but has a few procedural questions he guesses some of the more experienced here could know.

Ok so SWIM is making the gear using 20% BB, 2% BA, although after some time he will convert to 1.2% BA or so because he will have a more sterile environment etc.

So ok, SWIM is using the standard "researchsuplly" peristaltic pump with 0.22 filters BUT how in GODS name can SWIM fill those 10ml vials with 10ml of liquid CONSISTENTLY.

Its driving SWIM literally crazy:p

Using a syringe is pretty darn slow and in any case everything remains exposed for too long...

With regards

p.s. SWIM stands for Someone Who Isnt Me
 
for the love of god...how insanly stupid can SWIM be?

he was using a god damn pippete all that time, jeeeesus.

ok swim saw some dispensers at research supply, are these as cheap as they come?
 
you can use .45 mm filters too. They are a lot faster, and just as good
 
swim uses the 0.22, speed is not an issue with a peristaltic pump and the additional spores etc that are filtered out are worth it.

though this thread is concluded you have been VERY helpful:)
 
New to the board - but a chemist in real life - so let me add my $0.02

I would say that peristaltic pumps are almost *ideally* suited to this sort of work - any pump that relies on an air pressure differential - in my filtration experience - is at a distinct disadvantage with more viscous liquids like oils.

Filtration while pumping is good - just make your dispenser is sterile before starting - no point in contaminating nice sterile product on the final step!

Doctor9
 
what do you guys mean by dispenser exactly?

I looked on RLS and couldnt find anything called a dispenser...
 
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