Autoclaving as final sterilzation???

Hey bros,

after some research i have found that one of methods used to sterilize the hormones based in oils is to Autoclave it.
please check below:

"Example A 40% solution of testosterone enanthate is prepared :by dissolving 4.0 g. in benzyl benzoate to make ml. of final volume. After autoclaving at 121 C. for 2 hours to sterilize, 0.25 ml. of the solution is injected into the vastus lateralis muscle of the rabbit and the lesion size is measured after 2 days. A lesion consisting of 847 cubic millimeters is produced.
When this same quantity of hormone is dissolved in a vehicle consisting of benzyl benzoate and 80% sesame oil and 0.25 ml. is injected a lesion size of 1441 cubic millimeters is produced. "

Please notice that for sterilization purposes the autoclave was used (121 C for 2 hours).

How do you think is there any difference between autoclave and dry heat sterilization method (simple oven etc) at 121 celcius?- because the solution inside the vial is not effected by the saturated steam so would it be any difference if it would be just putted in oven for final sterilization?

Thank you!

i have no clue what the hell an auto clave is lol, but ill bump this for ya, i always though gear was as easy as making a cake, a little powder, a little benzle , some oil, shake, heat, shoot lol, guess its not that easy lol

autoclaving has a pressure element to it that makes quite a large difference. in addition an autoclave machine is pricey.

csuperman said:

autoclaving has a pressure element to it that makes quite a large difference. in addition an autoclave machine is pricey.

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yes , csuperman, i totally agree with you!

but the point of autoclave is to keep a pressure that the boiling temperature of water goes higher---> higher temperature water reaches---> faster the bacterias and all other bad things are destroyed.

but the boiling point of OILS is tottaly different story. it can easily reach 200 Celcius and even more (depends on the kind of oil).
So ones again it makes a little bit strange why the autoclave is used while it could be just putted in 121 celcius Oven and kept there for 2 hours.


And even if the vial would contain water instead of oil i am almost sure that this water would reach 110 celcius minimum before boiling or even much more because due to the capping of vial (in case it is made right) the gass inside vial will create a pressure inside and the boiling point of water will increase drastically (pressure-cooker effect). So in this case closed vials with etc winstrol suspension should be same possible to sterilize in oven (but this is only theoretically)

I would have to look into it, I have never tried nor spent the time to learn about autoclaving oils, as its predominantly used for surgical instruments and the like.

It can be used, but really unnecessary, .2micron filter gives you a sterile solution, nothing else needed. Sterile vials, wash with 90%-91% isopropyl alcohol, micro wave for 2-3 min.TAA-DAA. If I were to ever do it, thats probably how Id go about it.

A pressure cooker will do it as well, its just harder to control the temp, and takes longer cause it stays at 15psi, where-as the autoclave will go higher psi.

Maybe the 121 is based on hormone max heat and not the oil boiling?

My concerns would be pressure cooking sealed vial might break it???

Oh hi, I am new here...no exp. , just in learning/planning phase.. I am planning on pressure cooking all my beakers/grad cyls and tools (wrapped in foil,kept Out of the cooker water) before start.

Maybe overkill....only waste my effort....and I got a lot of that...



Edit

I haven't thought about the differences between an auto clave and pressure cooker for a while, since another project a long time ago before my internet access , so my curiosity was piqued and I read up on it and an additional factor in an autoclave is that it sucks to air out first and it starts under vacumm pressure. Maybe that is why sealed vials dont blow up, but might in a pressure cooker.

MuscleMaker said:

A pressure cooker will do it as well, its just harder to control the temp, and takes longer cause it stays at 15psi, where-as the autoclave will go higher psi.

Maybe the 121 is based on hormone max heat and not the oil boiling?

My concerns would be pressure cooking sealed vial might break it???

Oh hi, I am new here...no exp. , just in learning/planning phase.. I am planning on pressure cooking all my beakers/grad cyls and tools (wrapped in foil,kept Out of the cooker water) before start.

Maybe overkill....only waste my effort....and I got a lot of that...



Edit

I haven't thought about the differences between an auto clave and pressure cooker for a while, since another project a long time ago before my internet access , so my curiosity was piqued and I read up on it and an additional factor in an autoclave is that it sucks to air out first and it starts under vacumm pressure. Maybe that is why sealed vials dont blow up, but might in a pressure cooker.

Click to expand...

it would not break as vials are made from special glass which is able to stand a heat (i have tried this already in one laboratory).

the main questions still keeps a same- what is the difference between putting sealed vial into autoclave and putting it in simple heat oven?- because the liquid is already sealed and it would not be touched directly by saturated steam. So how do you think, is there any difference at all ?

Not breaking from heat, but from pressure is my concern.

as far as the difference between a pressurized wet heat and ambient dry heat...

at normal pressure, water and steam wont get any hotter than a little above boiling temp. cause as you add heat, the water turns to steam nstead of getting any hotter. under pressure, the water wont boil until a higher temp, so it can get hotter. this includes any water IN any bacteria in there. also the wet steam will conduct the heat into the glass and vial much quicker than hot air...so it would get to temp much quicker.

not sure which of of those factors is bigger, but suffice to say, pressurized wet heat is MUCH faster at killing stuff than not.

but one advantage a .2 filter has ,I think, is that it also filters some metabolites of the bac out, where as cooking it may kill the germs, but some of their toxic metabolites(their piss and crap) may still be there. this is why they use horseshoe crab blood to check a sample of pharma drugs AFTER sterilization. they can tell if that stuff is in there with it.

MuscleMaker said:

Not breaking from heat, but from pressure is my concern.

as far as the difference between a pressurized wet heat and ambient dry heat...

at normal pressure, water and steam wont get any hotter than a little above boiling temp. cause as you add heat, the water turns to steam nstead of getting any hotter. under pressure, the water wont boil until a higher temp, so it can get hotter. this includes any water IN any bacteria in there. also the wet steam will conduct the heat into the glass and vial much quicker than hot air...so it would get to temp much quicker.

not sure which of of those factors is bigger, but suffice to say, pressurized wet heat is MUCH faster at killing stuff than not.

but one advantage a .2 filter has ,I think, is that it also filters some metabolites of the bac out, where as cooking it may kill the germs, but some of their toxic metabolites(their piss and crap) may still be there. this is why they use horseshoe crab blood to check a sample of pharma drugs AFTER sterilization. they can tell if that stuff is in there with it.

Click to expand...

"also the wet steam will conduct the heat into the glass and vial much quicker than hot air...so it would get to temp much quicker."

that point is very true and i was looking behind it same but the fact is that in dry heat it would still reach the same 121 temperature pretty quickly (specially having that the liquid inside is only 10ml which is not too much to heat-up fast).