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Advanced Supplements for legit IGF-1 LR3
By Bryan Haycock
To understand how IGF-1 works you have to understand how muscles grow. The ability of muscle tissue to constantly regenerate in response to activity makes it unique. It’s ability to respond to physical/mechanical stimuli depends greatly on what are called satellite cells. Satellite cells are muscle precursor cells. You might think of them as "pro-muscle" cells. They are cells that reside on and around muscle cells. These cells sit dormant until called upon by growth factors such as IGF-1. Once this happens these cells divide and genetically change into cells that have nuclei identical to those of muscle cells. These new satellite cells with muscle nuclei are critical if not mandatory to muscle growth.
Without the ability to increase the number of nuclei, a muscle cell will not grow larger and its ability to repair itself is limited. The explanation for this is quite simple. The nucleus of the cell is where all of the blue prints for new muscle come from. The larger the muscle, the more nuclei you need to maintain it. In fact there is a "nuclear to volume" ratio that cannot be overridden. Whenever a muscle grows in response to functional overload there is a positive correlation between the increase in the number of myonuclei and the increase in fiber cross sectional area (CSA). When satellite cells are prohibited from donating new nuclei, overloaded muscle will not grow (Rosenblatt,1992 & 1994; Phelan,1997). So you see, one important key to unnatural muscle growth is the activation of satellite cells by growth factors such as IGF-1.
IGF-1 stimulates both proliferation (an increase in cell number) and differentiation (a conversion to muscle specific nuclei) in an autocrine-paracrine manner, although it induces differentiation to a much greater degree. This is in agreement with the Dual Effector theory. In fact, you can inject a muscle with IGF-1 and it will grow! Studies have shown that , when injected locally, IGF-1 increases satellite cell activity, muscle DNA content, muscle protein content, muscle weight and muscle cross sectional area (Adams,1998).
On the very cutting edge of research scientists are now discovering the signaling pathway by which mechanical stimulation and IGF-1 activity leads to all of the above changes in satellite cells, muscle DNA content, muscle protein content, muscle weight and muscle cross sectional area just outlined above. This research is stemming from studies done to explain cardiac hypertrophy. It involves a muscle enzyme called calcineurin which is a phosphatase enzyme activated by high intracellular calcium ion concentrations (Dunn, 1999). Note that overloaded muscle is characterized by chronically elevated intracellular calcium ion concentrations. Other recent research has demonstrated that IGF-1 increases intracellular calcium ion concentrations leading to the activation of the signaling pathway, and subsequent muscle fiber hypertrophy (Semsarian, 1999; Musaro, 1999). I am by no means a geneticist so I hesitated even bringing this new research up. In summary the researchers involved in these studies have explained it this way, IGF-1 as well as activated calcineurin, induces expression of the transcription factor GATA-2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of the transcription factor nuclear factor of activated T cells or NF-ATc1. Thus, IGF-1 induces calcineurin-mediated signaling and activation of GATA-2, a marker of skeletal muscle hypertrophy, which cooperates with selected NF-ATc isoforms to activate gene expression programs leading to increased contractile protein synthesis and muscle hypertrophy. Did you get all that?
By Author Unkown
IGF stands for insulin-like growth factor. It is a natural substance that is produced in the human body and is at its highest natural levels during puberty. During puberty IGF is the most responsible for the natural muscle growth that occurs during these few years. There are many different things that IGF does in the human body; I will only mention the points that would be important for physical enhancement. Among the effects the most positive are increased amino acid transport to cells, increased glucose transport, increased protein synthesis, decreased protein degradation, and increased RNA synthesis.
When IGF is active it behaves differently in different types of tissues. In muscle cells proteins and associated cell components are stimulated. Protein synthesis is increased along with amino acid absorption. As a source of energy, IGF mobilizes fat for use as energy in adipose tissue. In lean tissue,
IGF prevents insulin from transporting glucose across cell membranes. As a result the cells have to switch to burning off fat as a source of energy.
IGF also mimic's insulin in the human body. It makes muscles more sensitive to insulin's effects, so if you are a person that currently uses insulin you can lower your dosage by a decent margin to achieve the same effects, and as mentioned IGF will keep the insulin from making you fat.
Perhaps the most interesting and potent effect IGF has on the human body is its ability to cause hyperplasia, which is an actual splitting of cells. Hypertrophy is what occurs during weight training and steroid use, it is simply an increase in the size of muscle cells. See, after puberty you have a set number of muscle cells, and all you are able to do is increase the size of these muscle cells, you don't actually gain more. But, with IGF use you are able to cause this hyperplasia which actually increases the number of muscle cells present in the tissue, and through weight training and steroid usage you are able to mature these new cells, in other words make them grow and become stronger. So in a way IGF can actually change your genetic capabilities in terms of muscle tissue and cell count. IGF proliferates and differentiates the number of types of cells present. At a genetic level it has the potential to alter an individuals capacity to build superior muscle density and size.
There is a lot of talk about the similarity between IGF and growth hormone. The most often asked question is simply which is more effective. GH doesn't directly cause your muscles to grow, it works very indirectly by increasing protein synthesis capabilities, increasing the amount of insulin a person can use effectively, and increasing the amount of anabolic steroids a person can use effectively. GH also indirectly causes muscle growth by stimulating the release of IGF when it (the GH) is destroyed in the human body. So one way you could look at it as GH being a precursor to IGF. So to put it simple IGF is more effective at directly causing muscle growth and density increases. IGF is also much more cost effective.
IGF can also be effectively used by itself and gains will still be easily noticeable. With growth hormone you need to use high amounts of anabolics and often insulin to see any gains at all, this is not the case with IGF. IGF can be used by itself and is often used by bodybuilders who bridge between cycles, during this bridge is a good time to use IGF since it has no effect on natural testosterone production so it will therefore allow you to return to normal in terms of hormone levels. A stack of IGF, PGF2a, HCG, and clomid would be a good bridge stack and would allow your body to return to normal and still allow you to retain and make new gains.
IGF is a research drug, it hasn't been approved by the FDA for use as a pharmaceutical and it is currently being researched for nerve tissue repair, possible burn victims, and also as a possible aid in muscle wasting for AIDS patients. There are many different analogs of IGF available, instead of mentioning them all, I will simply mention the two most common and the most effective. Regular recombinant IGF is one of the two, it is also the more expensive and the least effective. Regular IGF only has a half-life of about 10-20 minutes in the human body and is quickly destroyed, it can be combined with certain binding proteins to extend the half-life, but it is not a very simple procedure and there is a more effective and less expensive version available. The most effective form of IGF is Long R3 IGF-1, it has been chemically altered and has had amino acid changes which cause it to avoid binding to proteins in the human body and allow it to have a much longer half life, around 20-30 hours. "Long R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substition of an Arg(R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF-1 has been produced with the purpose of increasing the biological activity of the IGF peptide."
"Long R3 IGF-1 is signifacantly more potent than IGF-1. The enhanced potency is due to the decreased binding of Long R3 IGF-1 to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGF's."
It is also not as expensive since a media grade version is available which is sufficient for bodybuilding use. There is also a receptor grade available but it is VERY expensive and the only noticeable difference between the two would only be able to be noticed in a laboratory setting. The price on the black market for Long R3 IGF-1 can be seen anywhere from $200-$500 per milligram depending on the source.
The most effective length for a cycle of IGF is 50 days on and 20-40 days off. The most controversy surrounding Long R3 IGF-1 is the effective dosage. The most used dosages range between 20mcg/day to 120+mcg/day. IGF is only available by the milligram, one mg will give you a 50 day cycle at 20mcg/day, 2mg will give you a 50 day cycle at 40mcg/day, 3mg will give you a 50 day cycle at 60mcg/day, 4mg will give you a 50 day cycle at 80mcg/day and so on. The dosage issue mainly revolves around how much money you have to spend, plenty of people use the minimum dosage of 20mcg/day and are happy with the results, and in fact several top bodybuilders use the 20mcg/day dosage and are pleased with the results. IGF is most effective when administered subcutaneously and injected once or twice daily at your current dosage. The best time for injections is either in the morning and/or immediately after weight training.
Another frequently asked question of IGF refers to the real world results, in terms of pure weight gain don't expect to gain 5 lbs. a week like you may off of anadrol or a similar steroid. The only weight you will gain from IGF use is pure lean muscle tissue, with steroids most of the weight gained is water weight. With an effective dosage you can expect to gain 1-2 lbs of new lean muscle tissue every 2-3 weeks and these effects can be increased with the use of testosterone, anabolic steroids, and insulin use. Increased vascularity is also very common, people report seeing veins appear where they never have before. And yet another effect reported is the ability to stay lean while bulking with heavy dosages of steroids and TONS of food while on an IGF cycle, this is perhaps the most pleasing effect. Increased pumps are also noticeable almost immediately, the pumps can almost become painful, pumps are even noticeable when doing cardio.
Overall, IGF is a very exciting drug due to its ability to alter ones genetic capabilities. If you can find a trustworthy source and you use it correctly it can be a VERY useful tool in your bodybuilding drug arsenal.
uhhhh did you do any research? and why only 2 kits of HGH? how many ius do you plan on runnning? have you ever used slin before? IGF before?
I am the irishman your mother warned you about.....
I dont have a girlfreind but i know a girl who would get really mad at me if she heard me say that...
Disclaimer - My comments in no way promote the use of androgenic/anabolic steroids and/or any prescription drug without a physician's prescription. All my comments, questions and referrals are striclty for entertainment purposes only!
IMO try Slin and maybe GH along with it before you start IGF and slin. Some notice extreme insulin sensitivity when taking IGF and I would hate for anything crazy to happen to you. 2 kits of gh won't do much since the minimum most recommend (including myself) is 3 months and prefered is more than 6 and at around 3-6iu per day. At 4iu ed that will give you 50 days and too short of a timeframe IMO.
Bump the other fellas on your experience and research. Just trying to help.
bumpin this up! IGF is great bruthaz I loved the results i got from it!
An IRA man shows up at the pearly gates and St. Peter comes out to greet him. St. Peter takes one look and says "I don't think you can get in here.The IRA man says"Who wants in? You've twenty minutes to get the *#!@ out!
Disclaimer - My comments in no way promote the use of androgenic/anabolic steroids and/or any prescription drug without a physician's prescription. All my comments, questions and referrals are striclty for entertainment purposes only!
"Among the effects the most positive are increased amino acid transport to cells, increased glucose transport, increased protein synthesis, decreased protein degradation, and increased RNA synthesis."
This is a nucleic acid polymar that consists of nucleotide monomers, that acts as a messenger between DNA and robisomes but is also responsible for making proteins out of amino acids. RNA polynucleotides contain ribose sugars and predominantly uracil unlike deoxyribonucleic acid or DNA, which contains Deoxyribose (da pimps shit in muscle building) and predominantly thymine which is a pyrimidine neucleoboase. As the name implies, thymine may be derived by methylation of uracil at the 5th carbon. Thymine is found in DNA. In RNA thymine is replaced with Uracil in most cases. In DNA, thymine binds to adenine via two hydrogen bonds (you feel the citric acid cycle coming on???? ok, seriously pay attention, I'm giving you the key to being fukcing huge) to assist in stabilizing the nucleic acid structures.. It is broken down from DNA by enzymes (raw foods baby) called RNA polymerases and further processed by other enzymes. RNA serves as the template for translation of genes into proteins, transferring amino acids to the ribosome to form proteins, and also translating the transcript into proteins. (in other words, MUSCLE)
As a source of energy, IGF mobilizes fat for use as energy in adipose tissue (fat). In lean tissue, IGF prevents insulin from transporting glucose across cell membranes. As a result the cells have to switch to burning off fat as a source of energy. This is also really sick because adipose tissue is relatively close to adenosene triphosphate in the citric acid cycle. Thymine comes into play now, Tymine is found in the nucleic acid DNA. In RNA, However, thymine is replaced with uracil in most cases. In DNA, thymine binds to adenine via two hydrogen bonds to assist in stabilizing the nucleic acid structures.
In other words, IGF-1A and MGF-1 (I know we haven't talked too much about this but pay attention anyway) makes muscles more sensitive to insulin's effects and nothing more. It makes the "receptor" or in other words DNA nucleotide, more suseptible to the effects of herplasia through it's transcription. What it's doing is rewriting your genetical mapping.
after puberty you have a set number of muscle cells, and up until this was understood you could NOT duplicate those cells, you where restricted by your genetics, BB'ers of the past diddn't actually gain more muscle, they pumped the cell up with ATP. But, with IGF use you are able to cause hyperplasia which actually increases the number of muscle cells present in the tissue and through weight training (intense weight training and strain) and anabolic usage you are able to mature these new cells, in other words make them increase in numbers and become larger.
GH doesn't DIRECTLY cause your muscles to grow, it works very indirectly by increasing protein synthesis capabilities, increasing the amount of insulin a person can use effectively without getting fat, and increasing the amount of anabolic steroids a person can use effectively.
"Long R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substition of an Arg(R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF-1 has been produced with the purpose of increasing the biological activity of the IGF peptide."
I'm going to quote the Human Genome Project now:
It's a project to de-code more than 3 billion nucleotides contained in a haploid reference human genome and to identify all the genese present in it. The reference human genome sequence was considered complete to human standards at 92% in the year 2005 (research began around 1972) and somewhat independently by Celera Gnomics (which we'll leave out of this but they are peices of shit). The "genome" of any given individual (except for twins and cloned animals) is unique; mapping "the human genome" involves sequencing multiple variations of each gene. The project did not study all of the DNA found in human cells; some heterochromatic areas (about 8% of the total) remain unsequenced."
So, you see......... understanding this sequence and the factors involved in the cause of hyperplasia is key to growth for muscle cells. Each individual muscle cell is stimulated by a certian amount of stress applied to the muscle and is then "directed" to either GROW or STRENGTHEN through the stimuli presented it by the chemical relay process delivered unto the receptor. The receptor sends the signal to the brain initiating the release of growth factors such as IGF and MGF which, in conjunction with anabolics will ensure the hypertrophy of the individual cell.
Food (protein in this case) + IGF + Insulin (transport system) converts to as close to a muscle cell as you're going to get, this bonds with existing cells and before you know it, millions of bonds are formed and you're fukcing huge.
The question is WHY????
Why does it do this? It's because the Human Genome is designed such that regrowth is ever more prevelant than maintenace. You're body chemistry is already mapped from before birth and when you remove any of those prevelant cells, either one at a time or several million, the bodies initial response is to return those and strengthen the weak point. We truly are ever growing and ever evolving.
I hope this has helped at least one person to understand the process, feel free to email me with questions.
Great thanks just wanted to make sure. If you don't mind me bothering you with questions. Nobody really talks about the side effects. Is IGF a Harmone? Will your intestine grow extremly large after awhile? I'm looking to lean out and gain muscle mass I way about 225 6'4 I would like to get 250 but lean. Do you think IGF is the way to go? Also IGF 1 IGF 2, IGF 3 Are these the same?
hey there. i am new to all of this stuff but had a buddy telling me about igf so i have been researching for about 2 weeks now and ordered some last night. i have some questions about it...on another board it talked about having to reconstitute it with acetic acid, is this true? the stuff i ordered comes with a vial of bacteriostatic water, can i not just use this? and what is the recommended dosage range for someone who has never taken this kind of stuff?
You should have ordered from us you wouldn't need bother reconstituting it as our is ready to go . Tryed true and proven
If you mix the bact water with the igf it will only be good for a few days bro at the most another words it will ruin it other the the first couple days worth after that it will be no good in bact water so u need to get aa solution or you can use ba and this will allow it to stay good refrigerated for long period of time where as the bact water will not
ok so i just ordered some igf from mc and am stoked about it. i know it comes premixed or whatever, but it needs to be stored in the frig right? and what you guys suggest is a good/safe start dose range? i am new to al of this stuff, so i need all the advice i can get. i know so far to use it bilaterally, but for how many days/week and how many weeks? stuff like that. i would appreciate all the help/advice i can get
Great Post I have learned a lot just on this post alone.
I was looking for a post from some of the advanced members on the diet for IGF.
I know it all depends on what one wants to accomplish with IGF. What is the correct diet to IGF. I know everyone is different. I think for the new comers to IGF a post with the correct diet would help.
With IGF are you suppose to double your calories? I always thought it should be 50 30 20 ratios. With a good work out plan 3-4 times a week. I know your not going to get big with IGF its more for a cutting diet. Correct? Thanks for you help.
Is this a proven fact bro? Not trying to give you a hard time, but was on bolex the other day and guys saying they have been using bac water for a long time. I used you guys for my IGF 1 R3 and who I spoke with who I spoke with had nothing but good things to say about this site and you Presser.
Thanks in advance. I bought some ph balanced aa that I will be using, just curious about the bac water issue.
only went 50-70mcg ed for 6 weeks.
I whanna pick some up soon and add it to my bulker, maybe as a bday gift to myself :-)
bulking or cutting its good, personally i dont need it to cut if i had to pick id use it in bulkers but i do think ill use in end of spring maybe also.
Ok so what is the final consensus of EXACTLY how many minutes before a workout to pin IGF? One member i read said one hour before workout,another said 40 min before workout, then protein/carb shake. Also, does it matter in my case where i do 20 min ofcardio before workout? Just trying to see what commonlyis working best for most.
If you're not using MC IGF then you're wasting your time and money. Super effective and easy to use with quality gains you will feel and see, other sites and claims are false along wtih the chance of F-ing it up when you mix it wrong, too much or use the wrong solution. Dont waste another dollar trying others unless you enjoy wasting your money and in that case please feel free to donate your cash to my supplement fund or my Non Profit "Bodybuilders for muscle gains" organization, every penny helps.
www.musclechemadvancedsupps.com is having our annual anniversary sale, so huge sales going on now!
Evening versus morning injections of growth hormone (GH) in GH-deficient patients: effects on 24-hour patterns of circulating hormones and metabolites.
Jørgensen JO1, Møller N, Lauritzen T, Alberti KG, Orskov H, Christiansen JS.
Since serum GH in normal subjects displays a circadian variation with a major and consistent surge after the onset of sleep we examined whether the time of GH administration in GH-deficient patients had any impact on its action. Eight GH-deficient patients all underwent 3 4-week study schedules in random order: 1) evening (2000 h) sc GH injections, 2) morning (0800 h) sc GH injections, and 3) no GH administration. At the end of each period the patients were admitted to hospital for 24-h measurements of hormones and metabolites. For comparison, 10 age- and sex-matched healthy untreated subjects were hospitalized once under identical conditions. Mean (+/- SE) GH availability, i.e. the area under the curve (AUC; micrograms per L/12 h) for 12 h after injection was significantly greater after evening injection than after morning injections [83.3 +/- 25.4 (evening) vs. 46.0 +/- 10.6 (morning); P less than 0.01]. This might be due to higher skin and sc temperatures when in bed. The 2000-0800 h AUC after evening injection was similar to the corresponding AUC in the reference group. Mean 24-h serum insulin-like growth factor-I levels (micrograms per L) were similar after evening (189.8 +/- 2) and morning (179.5 +/- 5.3) injections (P = 0.8), but the latter displayed a circadian variation suggesting that a steady state had not been reached. Both were significantly lower than the stable reference value (248.4 +/- 3.6 micrograms/L). Blood glucose profiles after morning and evening GH did not differ from that of the reference group, whereas blood glucose decreased when the patients received no GH (P less than 0.01). Daytime (0800-2400 h) insulin levels were increased after morning injections (P less than 0.05). Nighttime levels of lipid intermediates were below normal in the untreated state and after morning injection (P less than 0.05), whereas nighttime blood alanine tended to be above normal after morning GH injection (P = 0.08). Highly significant inverse relationships between circadian lipid intermediates and both blood alanine and lactate concentrations were observed in the reference group and in the patients after evening injections. These relationships disappeared after morning injections. We conclude that the metabolic effects of sc GH injections are clearly influenced by the time of administration and that the closest similarity to normal hormone and metabolite patterns and relationships is reached by GH injection in the evening in GH-deficient patients.