Gear and contamination?

[Pharm_Fed]

I have two questions regarding this issue.

1) No matter how slowly I pull, I always get air in the syringe. So to draw the right amount I always overdraw to compensate for the air, the push it back into the vial. Sometimes, I actually have too much oil too so there is some oil that makes it into the syringe and then gets put back in the vial. I assume this isn't a huge deal because the syringe should be sterile. Just making sure it's nothing to worry about.

2) This just happened the first time last night. Was pulling oil into the syringe and air bubble starting coming up in the vial. It appeared as though air was entering the vial between the rubber stopper and the outside of the needle.I guess I may have had the needle at too much of an angle? I hope that's clear, it's kind of hard to explain. Is this a problem, or nothing to worry about?

Thanks.

 

[nuknuk]

what gauge needle are you using to draw with?

 

[h8tr3d]

if you can, draw with a 21, or even 23. I use a 25, but it take forever, and you get little bubbles, that take a while to dissipate. 21, or 23, cant go wrong

 

[h8tr3d]

also, always swab the rubber stopper with alcohol, before sticking the draw pin in, and always use a new draw pin. Pins are cheap, abcesses are not...... good rule of thumb

 

[big lifter]

1) syringes are sterile, so no need to worry about drawing too much and reinjecting back into the vial.

2) this happens when you keep drawing from the air tight vial untill there is too much vacuum inside, so it will compensate by sucking air in when there is even a tiny opening, like u said from the needle hole. fix this by shooting say 2ml of air into the vial before drawing ur shot, if its a 2ml shot. sort of to keep the blance.

 

[Pharm_Fed]

what gauge needle are you using to draw with?

22G

also, always swab the rubber stopper with alcohol, before sticking the draw pin in, and always use a new draw pin. Pins are cheap, abcesses are not...... good rule of thumb

Yeah, I always use an alcohol pad on the stopper and always use a new pin. Definitely better safe than sorry.

1) syringes are sterile, so no need to worry about drawing too much and reinjecting back into the vial.

2) this happens when you keep drawing from the air tight vial untill there is too much vacuum inside, so it will compensate by sucking air in when there is even a tiny opening, like u said from the needle hole. fix this by shooting say 2ml of air into the vial before drawing ur shot, if its a 2ml shot. sort of to keep the blance.

Yeah, I always inject air in the vial to compensate, don't know why this time the bubbles formed. Maybe I will inject a little bit more air in the vial than I take out.

Thanks

 

[superted]

22G



Yeah, I always use an alcohol pad on the stopper and always use a new pin. Definitely better safe than sorry.



Yeah, I always inject air in the vial to compensate, don't know why this time the bubbles formed. Maybe I will inject a little bit more air in the vial than I take out.

Thanks

I always do that