Myostatin inhibator coming soon!
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hitmeoff
New member
Ok guys, I got a Myostatin inhbitor that my rats have been using for about a week and a half now.
Its not an oral like MyoBlast or anything crappy like that. Its an injectable, shot everyday Sub-Q.
The preparation for this stuff is kinda complicated, you gotta basically reconstitue 3 different kinds of siRNAs then mix them together and distribute them into smaller vials that hold approx 2ml of solution. Your rats use 1ml a day.
It seems to be doing something. Ever since my rats got a cold about a month ago they were loosing strength, even when they were a few weeks removed from the cold they was still suffering strength loss (they had also just come off a HEAVY HEAVY cycle about 2 months ago). But last week was a VERY good week for them in the rat-gym. THey reagained all the strength they lost when they got sick, and gained some more on top of that. Thier weight has gone up about 2lbs as well.
A friend of mine used this on his rats before and apparently gained 11lbs over the course of 2 months...thats a big rat! So this doesnt seem like a magic bullet or anything like that, but the it does seem to give some results (it did for my buddy's rats, and it SEEMS to be doing it for my rats too).
Its not an oral like MyoBlast or anything crappy like that. Its an injectable, shot everyday Sub-Q.
The preparation for this stuff is kinda complicated, you gotta basically reconstitue 3 different kinds of siRNAs then mix them together and distribute them into smaller vials that hold approx 2ml of solution. Your rats use 1ml a day.
It seems to be doing something. Ever since my rats got a cold about a month ago they were loosing strength, even when they were a few weeks removed from the cold they was still suffering strength loss (they had also just come off a HEAVY HEAVY cycle about 2 months ago). But last week was a VERY good week for them in the rat-gym. THey reagained all the strength they lost when they got sick, and gained some more on top of that. Thier weight has gone up about 2lbs as well.
A friend of mine used this on his rats before and apparently gained 11lbs over the course of 2 months...thats a big rat! So this doesnt seem like a magic bullet or anything like that, but the it does seem to give some results (it did for my buddy's rats, and it SEEMS to be doing it for my rats too).
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einstein1905
New member
MyoGrow, as cheesey as the name sounds, is the only sound theoretical approach I've seen so far. They, as mentioned above, are implementing siRNA moeities that will bind to the mRNA encoding myostatin and effectively preventing translation of the myostatin mRNA into the myostatin protein. Gene "silencing" via siRNA is a powerful and rapidly evolving tool in molecular biology that has tons of potential. The beauty of it is that the siRNAs are injected, preferably IM, but it was mentioned sub Q above, and some will be taken up into cells where it will bind/pair with what it is designed to be complementary to, in this case myostatin mRNA...thus forming double stranded RNA. Since the only time the human body should be encountering dsRNA is when it is of a viral source, the body's quickly recognizes and degrades the dsRNA (consisting of the myostatin mRNA and the siRNA). This effectively prevents the myostatin protein from being synthesized. However, this system is beautiful because as the siRNA which has been injected pairs with myostatin mRNA and degrades and eventually runs out (gets titrated), the myostatin gene, GDF-8 (I believe) continues transcribing myostatin mRNA. Therefore, once there is no longer any siRNAin the body, pretherapy physiology ensues....no permanent disruptions in gene expression.
Some problems I have with their MyoGrow are that the effects will be localized. RNA is incredibly prone to degradation. It is very sensitive to fluctuations in temp and pH as well as salt conditions. In addition, RNAses are present everywhere. Injecting subQ only slows in the siRNA getting to where it needs to go...the muscle cells. The further away from its intended target you introduce it, the greater the chance of degradation. Also, the makers state that 12 nanomoles are sufficient for a 60 day in vivo human trial (hypothetically, of course). This was directly from their response to one of my emails directed to their tech people. They did not comment on the suggested frequency of injections. However, I doubt that this amt will be sufficient to have significant effects....but I may test that theory anyway
They offered no data to support that the siRNAs that they have developed will effectively "silence" myostatin but rather directed me towards general siRNA studies, with which I'm already very familiar.
I'm hesitant to say "MyoGrow" will or will not work, BUT it, in no way, should be compared to these BS myostatin blocking pills or other compounds...there simply is no validity to even a slight possibility that these products will selectively inhibit myostatin in any way.
I do take my hat off to the "MyoGrow" guys for using the most cutting edge and appropriate approaches to creating an effective inhibitor, but whether or not the specific siRNAs they have work is yet to be shown...to me anyway.
On a side not, a permanent myostatin inhibitor is relatively easy to desine, being that they have isolated myostatin and know it's structure. It's finding an effective way to reverse the process that will cause the delay.
Here's a link to the MyoGrow stuff. I am anxious to give it a try, but I'd want to run it alone or with something I already know how I react to, so that I can accurately guage its effect. I'd also like a little more scientific responses from the makers when responding to my emails. I will try it..withing 6 months or so.
Some problems I have with their MyoGrow are that the effects will be localized. RNA is incredibly prone to degradation. It is very sensitive to fluctuations in temp and pH as well as salt conditions. In addition, RNAses are present everywhere. Injecting subQ only slows in the siRNA getting to where it needs to go...the muscle cells. The further away from its intended target you introduce it, the greater the chance of degradation. Also, the makers state that 12 nanomoles are sufficient for a 60 day in vivo human trial (hypothetically, of course). This was directly from their response to one of my emails directed to their tech people. They did not comment on the suggested frequency of injections. However, I doubt that this amt will be sufficient to have significant effects....but I may test that theory anyway
They offered no data to support that the siRNAs that they have developed will effectively "silence" myostatin but rather directed me towards general siRNA studies, with which I'm already very familiar.
I'm hesitant to say "MyoGrow" will or will not work, BUT it, in no way, should be compared to these BS myostatin blocking pills or other compounds...there simply is no validity to even a slight possibility that these products will selectively inhibit myostatin in any way.
I do take my hat off to the "MyoGrow" guys for using the most cutting edge and appropriate approaches to creating an effective inhibitor, but whether or not the specific siRNAs they have work is yet to be shown...to me anyway.
On a side not, a permanent myostatin inhibitor is relatively easy to desine, being that they have isolated myostatin and know it's structure. It's finding an effective way to reverse the process that will cause the delay.
Here's a link to the MyoGrow stuff. I am anxious to give it a try, but I'd want to run it alone or with something I already know how I react to, so that I can accurately guage its effect. I'd also like a little more scientific responses from the makers when responding to my emails. I will try it..withing 6 months or so.
hitmeoff said:
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hitmeoff
New member
whiteox said:
LOL...you know with everything I give to my rats, I usually do ALOT and I do mean ALOT of research on. I usually soak up all the information I can gather about drugs, then decided whether or not to use them on my rats. With this one, one of my buddies said: "Im testing this new Myostatin blocker my buddy's company created, in 6 weeks my rats are up 10lbs" So I said "Cool, how can I get it?"
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hitmeoff
New member
One more note on this guys.
I forgot to mention that Silobiotech, of course, does not sell this stuff for bodybuilding use. This is strictly for research purposes only and we should refer to it in such a fashion.
But Im sure you guys knew that since we are always conducting experiments on our lab rats.
I forgot to mention that Silobiotech, of course, does not sell this stuff for bodybuilding use. This is strictly for research purposes only and we should refer to it in such a fashion.
But Im sure you guys knew that since we are always conducting experiments on our lab rats.
hitmeoff
New member
whiteox said:Hitmeoff - you must have some huge rats
What about what einstein said? Why are you giving your rats sub-q instead of IM? Keep us posted on your gains - sounds like you're happier (at least initially) with this stuff then the lipostabil.
Yes, these things are bigger than NY City Sewer rats! :thumbsup:
I had been giving my rats Sub-Q injections because thats how my friend was using it when he was experimenting on his rats.
However after talking on PM with einstein and after emailing Silobiotech with some questions, apperently this stuff is much more efficient if its injected IM. So this morning I gave my rats an injection by IM. They squirmed alot, about the same as thier sub-q shots...I assume it hurt them alot...something burning not much unlike thier squirming on IPs Prop/Susp mix. But they seem to be moving gingerly right now, so I can assume they arent sore at all like they would be on a Prop/Suspension shot.
Okay, so what I gather from this thread is that Myogrow works best with IM injects. Also that the effects are localized? If thats the case, doesn't that mean that if you do a typical delt injection, that delt will be freakishly bigger than the other? Also whats the difference between Myogrow and GASP-1? If I wanted to try this on myself, is 1 ML enough given that "12 nanomoles are sufficient for a 60 day in vivo human trial" (4 for rats). I'm going to be getting this stuff soon and I want to do this right, Thanks
einstein1905
New member
Moe4me said:
First off, no, a shot to one delt and not the other won't make you freakishly asymmetric-it's not like synthol. It's not going to have immediate and huge effects. I also would recommend alternating sides when you spot inject anyway.
GASP-1 is a protein that's been shown (in one or maybe two studies) to bind myostatin and negate its effects. I haven't read the full paper yet, but that could simply mean a 10% inhibition of myostatin's effects. All you need is statistically significant inhibition, and then you can title your paper, "compound X inhibits compound Y". Myogrow (in theory and very possibly in reality) inhibits at the nucleic acid level...it inhibits myostatin from even becoming a protein. The advantage of Myogrow (siRNA silencing) over using a purified GASP-1 (protein) is that Myogrow has the better potential to fully inhibit myostatin. However, RNA is very easily degraded, whereas protein is quite a bit more stable, which gives GASP-1 an advantage in that regard. I'll read the GASP-1 one paper in full and see what the numbers say.
Myogrow doesn't seem to be a very big investment for all of its possible potential...we'll wait and see how things work out with Hitmeoff. I may be next to try it.
hitmeoff
New member
Ok my experiment is going ot be a bit skewed...
In classic HMO style, my rats are doing more drugs than they should! LOL.
SO this week my rats started a CKD diet, and started doing cardio and started taking some ECA. Obviously all 3 factors are going to negatively impact muscle growth, right? right.
Well this week they also started taking 20mg ED of methyl-1-test.
Heres the thing, as of a week ago, my rats had gained about 2lbs after a 2 week period. Doesnt seem like much, but is a gain and im convinced it was totally lean gain because the rats looked a bit more toned.
Well 1 week after starting CKD, cardio, ECA and methyl-1-test and 3 weeks after starting myogro, my rat's initial weight gain is holding steady! Earlier in the week the rats weight dropped about 7lbs (obviously a loss of water from the glycogen loss) however, even at this lower weight, my rats managed to GAIN strength in thier workouts...not much, but it was there. My rats also seemed to be getting nice pumps...probably the methyl-1-test working there.
My rats finally carbed up on friday and today. The weight shot back up to the 7lbs they lost, however my rats' pants are fitting about as loose as they did some 25 odd pounds ago. Arm measurements held steady too.
So...I think the myogro is having a DEFINITE impact on my rats muscle growth as experianced by the sudden drop in weight YET gains in strength, despite being carb depleted (remember myogro growth appears to be slow and steady).
The addition of Methyl-1-test I think potentiate the effects of the myogro. Remember, a myostatin inhibitor's job is to stop the myostatin protien...the protien that basically limits how much your muscles can grow. If you effectively block this protien, its basically like a green light to your muscles to keep growing...but they still need the stimulation to do so...which is what AAS helps in doin. SO i think the combo is now really kicking in nicely!
In classic HMO style, my rats are doing more drugs than they should! LOL.
SO this week my rats started a CKD diet, and started doing cardio and started taking some ECA. Obviously all 3 factors are going to negatively impact muscle growth, right? right.
Well this week they also started taking 20mg ED of methyl-1-test.
Heres the thing, as of a week ago, my rats had gained about 2lbs after a 2 week period. Doesnt seem like much, but is a gain and im convinced it was totally lean gain because the rats looked a bit more toned.
Well 1 week after starting CKD, cardio, ECA and methyl-1-test and 3 weeks after starting myogro, my rat's initial weight gain is holding steady! Earlier in the week the rats weight dropped about 7lbs (obviously a loss of water from the glycogen loss) however, even at this lower weight, my rats managed to GAIN strength in thier workouts...not much, but it was there. My rats also seemed to be getting nice pumps...probably the methyl-1-test working there.
My rats finally carbed up on friday and today. The weight shot back up to the 7lbs they lost, however my rats' pants are fitting about as loose as they did some 25 odd pounds ago. Arm measurements held steady too.
So...I think the myogro is having a DEFINITE impact on my rats muscle growth as experianced by the sudden drop in weight YET gains in strength, despite being carb depleted (remember myogro growth appears to be slow and steady).
The addition of Methyl-1-test I think potentiate the effects of the myogro. Remember, a myostatin inhibitor's job is to stop the myostatin protien...the protien that basically limits how much your muscles can grow. If you effectively block this protien, its basically like a green light to your muscles to keep growing...but they still need the stimulation to do so...which is what AAS helps in doin. SO i think the combo is now really kicking in nicely!
sweatmachine
New member
Hell I'll try it,! I'm one crazy MoFo !
I once put a pickle in my nose....Im F'ing crazy like that!
BRING IT ON
I once put a pickle in my nose....Im F'ing crazy like that!
BRING IT ON
whiteox
New member
I think hitmeoff temporarily stopped taking it. He'll probably continue later. I'm doing my third inject (3rd day) of myogro tonight. Yes - i'd definately wait to hear our results before putting money into it. I'll post my results as the myogro is the only addition to my supps. in the last few months so if I notice anything it'll be the myogro.
Anyway, i'll keep you posted every week or so - only done 2 shots so far so I can't tell anything. This stuff may do something - and it may do nothing - stay tuned.
Anyway, i'll keep you posted every week or so - only done 2 shots so far so I can't tell anything. This stuff may do something - and it may do nothing - stay tuned.
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